Hydroxyproline Colorimetric Assay Kit

AgK-424

Hydroxyproline Colorimetric Assay Kit

Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. It is an easy, convenient method which results in a chromogen with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-2 μg.

Hydroxyproline (4-hydroxyproline) is a common nonproteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders.

The assay is useful over the range of 0.1-2 μg.

Store at 4°C.

100 assays

• Oxidation Buffer
• Chloramine T Concentrate
• Perchloric acid/Isopropanol Solution
• DMAB Concentrate (in DMSO)
• Hydroxyproline Standard (1 mg/ml)

96-well clear plate with flat bottom
Multi-well spectrophotometer
For hydrolysis: Polypropylene Vials and Screw Caps

Absorbance (560 nm)

Animal tissues; protein/peptide hydrolysates; serum; urine