Total Collagen Assay Kit (Colorimetric)

AgK-448

Total Collagen Assay Kit (Colorimetric)

Collagen Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore (Abs 560 nm). The assay is simple, sensitive and specific for collagen and can detect as low as 0.5 µg of collagen in a variety of samples such as tissue homogenates, biological fluids and purified proteins.

Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals. The triple-helical structure of collagen is quite unique: it consists of a repeating pattern of a basic trimer: Glycine-Proline-Hydroxyproline. In cells, collagens are secreted as procollagens and these chains are transported into the Endoplasmic Reticulum, where, numerous post-translational modifications lead to the formation of a triple helix with disulfide bonds. Excessive production of collagen is linked to pathological conditions including liver cirrhosis, lung fibrosis, and tumor growth.

Measurement of collagen in various sample types.

Store at -20°C.

100 assays

• Oxidation Buffer
• Chloramine T Concentrate
• Perchloric Acid/Isopropanol Solution
• DMAB Concentrate (in DMSO)
• Collagen I Standard (2 mg/ml)

96-well clear plate with flat bottom
Multi-well spectrophotometer
Hydrochloric Acid (concentrated)
Acetic Acid (Glacial)
For hydrolysis: Polypropylene Vials and Screw Caps

Absorbance (OD = 560 nm).

Mammalian tissues
Protein/peptide hydrolysates
Serum; Urine