Product Name
Erwinia amylovora Positive Control (10)
Product Overview
Erwinia amylovora Positive Control (10) is designed for the detection of Erwinia amylovora.
Storage
Our DAS-ELISA reagents are standardized for use at a dilution of 1:200 and a test volume of 200 μl/well. The products must be kept refrigerated (ca. 4°C) upon receipt. Once opened, we recommend using the reagents within 13 months.
Scientific Background
During the first step of the assay the surface of a microtiter plate is coated with the antigen-specific coating-antibody (IgG). When an antigen-containing sample is added during the second step, the antigen binds to the immobilized IgG, forming an antibody-antigen complex. This complex reacts with the enzyme-labelled antibody-AP-conjugate during the third step by forming a double-antibody sandwich. During the fourth step the alkaline phosphatase (AP) reacts with the substrate 4-nitrophenylphosphate in an enzymatic reaction, resulting in yellow coloured 4-nitrophenol as product. This colour development can be evaluated visually or measured in a spectrophotometer at 405 nm after 1 and 2 hours.
Detection method
DAS ELISA
Sample Type
Apple, pear, quince, other rosaceous trees and shrubs.
Datasheet 
