Product Name
Pantoea stewartii ssp. stewartii Negative Control (10)
Product Overview
Pantoea stewartii ssp. stewartii Negative Control (10) is designed for the detection of Pantoea stewartii ssp. stewartii.
Storage
Our DAS-ELISA reagents are standardized for use at a dilution of 1:200 and a test volume of 200 μl/well. The products must be kept refrigerated (ca. 4°C) upon receipt. Once opened, we recommend using the reagents within 14 months.
Scientific Background
During the first step of the assay the surface of a microtiter plate is coated with the antigen-specific coating-antibody (IgG). When an antigen-containing sample is added during the second step, the antigen binds to the immobilized IgG, forming an antibody-antigen complex. This complex reacts with the enzyme-labelled antibody-AP-conjugate during the third step by forming a double-antibody sandwich. During the fourth step the alkaline phosphatase (AP) reacts with the substrate 4-nitrophenylphosphate in an enzymatic reaction, resulting in yellow coloured 4-nitrophenol as product. This colour development can be evaluated visually or measured in a spectrophotometer at 405 nm after 1 and 2 hours.
Detection method
DAS ELISA
Sample Type
Poaceae, especially maize.
Datasheet 
