Cladosporium cladosporioides End-Point PCR Control (100)

CPD-575

Cladosporium cladosporioides End-Point PCR Control (100)

Cladosporium cladosporioides End-Point PCR Control (100) is designed for the detection of Cladosporium cladosporioides DNA based on the use of end-point PCR technology.

Cladosporium cladosporioides End-Point PCR Control (100) is designed for the detection of C. cladosporioides specific DNA based on the use of end-point PCR technology. This kit is designed for research use only and not for use in diagnostic procedures. The kit includes Master Mix and primers for the specific amplification of a 320 nucleotide region of the C. cladosporioides genome, as well as a positive control and a negative control to confirm the integrity of the kit reagents. In addition, the kit contains loading dye and a DNA ladder to facilitate analysis of the results.

All kit components should be stored at -20°C upon arrival;
Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots;
All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

Component Product
2X PCR Master Mix 350 μL
C. cladosporioides Primer Mix 70 μL
C. cladosporioides Positive Control 50 μL
Nuclease-Free Water 1.25 mL
Loading Dye 100 μL
DNA Ladder 100 μL
Product Insert 1

Appropriate Real-Time PCR Instrument with FAM and HEX filter channel;
DNA Purification Kit: The kit is compatible with all DNA purification kits that yield high quality, inhibitor-free DNA;
Disposable powder-free gloves;
Benchtop microcentrifuge;
Micropipettors;
Sterile pipette tips with filters;
PCR tubes;
Vortex mixer;
Agarose gel electrophoresis apparatus;
UV transilluminator with suitable gel documentation system;
PCR reaction preparation station (Optional).

Cladosporium is one of the most widespread molds. It includes about 40 species naturally found in soil, on decaying plant material and as plant pathogens. Cladosporium rot (Cladosporium spp.) of grapevine (Vitis vinifera) is a common disease, particularly in Cabernet Sauvignon and other red wine grape cultivars. It is favored by delayed harvest to obtain the phenolic maturity necessary for high-quality red wine. Symptoms appear on mature grapes and are characterized by berry dehydration, a firm decay affecting a small portion of the berry and a superficial olivegreen mold. Rapid and accurate detection of Cladosporium infections is highly important to facilitate the monitoring of Cladosporium in plant samples.

End-Point PCR

Plant tissues