Aspergillus niger End-Point PCR Detection Kit (24)

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Aspergillus niger End-Point PCR Detection Kit (24)

Aspergillus niger End-Point PCR Detection Kit (24) is designed for the detection of Aspergillus niger DNA based on the use of end-point PCR technology.

Aspergillus niger End-Point PCR Detection Kit (24) is designed for the detection of A. niger specific DNA based on the use of end-point PCR technology. The kit includes Master Mix and primers for the specific amplification of a 363 nucleotide region of the A. niger genome, as well as a positive control and a negative control to confirm the integrity of the kit reagents. In addition, the kit contains loading dye and a DNA ladder to facilitate analysis of the results.

All kit components should be stored at -20°C upon arrival;
Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots;
All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

Component Product
2X PCR Master Mix 350 μL
A. niger Primer Mix 70 μL
A. niger Positive Control 50 μL
Nuclease-Free Water 1.25 mL
Loading Dye 100 μL
DNA Ladder 100 μL
Product Insert 1

Appropriate Real-Time PCR Instrument with FAM and HEX filter channel;
DNA Purification Kit: The kit is compatible with all DNA purification kits that yield high quality, inhibitor-free DNA;
Disposable powder-free gloves;
Benchtop microcentrifuge;
Micropipettors;
Sterile pipette tips with filters;
PCR tubes;
Vortex mixer;
Agarose gel electrophoresis apparatus;
UV transilluminator with suitable gel documentation system;
PCR reaction preparation station (Optional).

Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus. It causes a disease called black mold on certain fruits and vegetables such as grapes, onions, and peanuts, and is a common contaminant of food. It causes stem rot of Dracaena, root stalk rot of Sansevieria and boll rot of cotton. It has also been implicated in the spoilage of cashew kernels, dates, figs, vanilla pods and dried prunes. It is ubiquitous in soil and is commonly reported from indoor environments, where it produces characteristic black colonies. Some strains of A. niger have also been reported to produce potent mycotoxins called ochratoxins. Methods for the rapid and sensitive detection of the pest would be valuable to ensure food quality and protection of individuals from the possibility of ingesting potentially hazardous myco- or ochratoxins.

End-Point PCR

Plant tissues