C58C1 Chemically Competent Cell

ACC-119

C58C1 Chemically Competent Cell

The genotype of C58C1 Chemically Competent Cell is Agrobacterium rhizogenes (str^R,rif ^R) pRiA4b (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The C58C1 Agrobacterium rhizogenes strain contains pRiA4b agrobacterium-type Ri plasmid with a broad host range (rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco, etc.) and streptomycin and rifampicin resistance.

C58C1 Chemically Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.

1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.

Store at - 80 °C for 12 months.

C58C1: 100 μL/tube * 10 tube/50 tube/100 tube.

Transformation efficiency of C58C1 Chemically Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.