Ar.1193 Chemically Competent Cell

ACC-120

Ar.1193 Chemically Competent Cell

The genotype of Ar.1193 Chemically Competent Cell is Agrobacterium rhizogenes (carb^R,str^R,rif ^R) pRi1193 (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The Ar.1193 Agrobacterium rhizogenes strain contains pRi1193 agrobacterium-type Ri plasmid with a broad host range (leguminosae, solanaceae, etc.) and carbenicillin, streptomycin, rifampicin resistance.

Ar.1193 Chemically Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.

Store at - 80 °C for 12 months.

Ar.1193: 100 μL/tube * 10 tube/50 tube/100 tube.

Transformation efficiency of Ar.1193 Chemically Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.