MSU440 Electroporation Competent Cell

ACC-124

MSU440 Electroporation Competent Cell

The genotype of MSU440 Electroporation Competent Cell is Agrobacterium rhizogenes(str^R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid with a broad host range (corn, tobacco, tea tree, artemisia annua, etc.) and streptomycin resistance. This competent cell is particularly suitable for the transformation of large plasmids.

MSU440 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.

1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.

Store at - 80 °C for 12 months.

MSU440 Electroporation: 50 μL/tube * 10 tube/50 tube.

Transformation efficiency of MSU440 Electroporation Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA.