K599 Electroporation Competent Cell

ACC-127

K599 Electroporation Competent Cell

The genotype of K599 Electroporation Competent Cell is Agrobacterium rhizogenes (str ^R) pRi2659 (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The K599 Agrobacterium rhizogenes strain contains pRi2659 agrobacterium-type Ri plasmid with a broad host range (cucurbitaceae, leguminosae, solanaceae, etc.) and streptomycin resistance. This competent cell is particularly suitable for the transformation of large plasmids.

K599 Electroporation Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.

1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.

Store at - 80 °C for 12 months.

K599 Electroporation: 50μL/tube * 10 tube/50 tube.

Transformation efficiency of K599 Electroporation Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^5 cfu/μg DNA.