TOP10 Chemically Competent Cell

ACC-129

TOP10 Chemically Competent Cell

The genotype of TOP10 Chemically Competent Cell is F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG, which is highly similar to DH10B (DH10B is galE15 and TOP10 is galU). The TOP10 strain, derived from MC1061 strain, is one of the most commonly used competent cells in the laboratory. TOP10 grows faster (faster than DH5 alpha, but slower than mach1-t1). The clone will be visible in 10 h. The mutations of recA1 and endA1 are beneficial to the stability of inserted DNA and the extraction of high purity plasmid DNA. The TOP10 Chemically Competent Cell can be used in cloning, blue and white spot screening, and has streptomycin resistance.

Store at - 80 °C for 12 months.

TOP10: 100 μL/tube * 20 tube /100 tube;

Transformation efficiency of TOP10 Chemically Competent Cell using pUC19 plasmid with 50 μg/mL kan is >10^8 cfu/μg DNA.