Phyto Casein Hydrolysate Yeast Extract Agar Base

MSM-003

Phyto Casein Hydrolysate Yeast Extract Agar Base

It contained 2.00g/L of Yeast extract, 4.00g/L of Casein hydrolysate, 10.00g/L of Glucose(anhydrous), 0.15g/L of Magnesium sulphate(anhydrous), 1.00g/L of Boric acid, 1.00g/L of Ammonium chloride, 1.20g/L of Tris HCl and 18.00g/L of Agar with the Final pH7.4 (at 25°C)

Suspend 37.35 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize both the solutions separately by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add the contents of one vial of CNP supplement. Mix well and pour into sterile Petri plates.

Clavibacter michiganensis subsp. michiganensis is a Gram-positive bacterium that causes wilting and cankers, leading to severe economic losses in commercial tomato production worldwide. Bacterial canker is one of the most destructive tomato diseases. Symptoms include necroses on leaves, stems and fruits during flowering of the plants.
(1) It can be recovered on any general microbiological media such as nutrient agar, however specialized medium such as Phyto Casein Hydrolysate Yeast Extract Agar Base are more advantageous. (2). It is comparatively a less selective medium.
Casein hydrolysate and yeast extract supplies nitrogeneous compounds. Glucose serves as a source of carbohydrate. Boric acid helps in selectivity of the medium. Tris HCL helps in buffering of the medium.

Cream to yellow coloured, homogeneous, free flowing powder

Firm, comparable with 1.8% Agar gel

Yellow coloured opalescent gel with white precipitate forms in Petri plates

Reaction of 3.77% w/v aqueous solution is pH 7.4 at 25°C

Cultural characteristics observed with added CNP supplement, after an incubation at 25-30°C for 6-7 days.

Semi selective medium for the detection of Clavibacter michiganensis subsp michiganensis on seeds of tomato.

Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.

1. Bradbury, J.F.(1986) Guide to plant pathogenic bacteria. CAB International Wallingford, UK.
2. Fatmi, M., Schaad, N.W. 1988. Semiselective agar medium for isolation of Clavibacter michiganensis subsp. michiganensis from naturally infected tomato seeds.