Phyto Cmm Agar Base

MSM-004

Phyto Cmm Agar Base

It contained 0.50g/L of Potassium dihydrogen phosphate, 2.00g/L of Di-potassium hydrogen phosphate, 0.122g/L of Magnesium sulphate anhydrous , 1.50g/L of Boric acid, 0.10g/L of Yeast extract, 10.0g/L of Sucrose and 18.00g/L of Agar with the Final pH7.3 (at 25°C)

Suspend 32.2 grams in 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize the medium by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add the rehydrated contents of one vial of CNNP supplement. Mix well and pour into sterile Petri plates.

Bacterial canker is a serious and destructive disease caused by Clavibacter michiganensis subsp. michiganensis in tomatoes and is of major concern due to the great losses to the tomato growing countries.
(1) Symptomatic plants showed reddish brown cavities in the stem, discoloration, and water soaking of vascular tissue. Diseased tissues were washed with phosphate buffer and placed on semi-selective Clavibacter medium (2), yeast extract serves as a nitrogenous source. Boric acid helps in the selectivity of the medium. Phosphates in the medium help in buffering of the medium. Sucrose serves as a carbohydrate source.

Light yellow coloured, homogeneous, free flowing powder

Firm, comparable with 1.8% Agar gel

Yellow coloured, opalescent gel forms in Petri plates

Reaction of 3.22% w/v aqueous solution is pH 7.3 at 25°C

Cultural characteristics observed with added CNNP supplement, after an incubation at 25-30°C for 6-7 days.

Semi selective medium for the detection of Clavibacter michiganensis subsp michiganensis on seeds of tomato.

Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.