Urine Exosome Purification and RNA Isolation Midi Kit

PE009

Urine Exosome Purification and RNA Isolation Midi Kit

The kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes ranging from 2 mL to 10 mL. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.

50-100 μL

35-40 minutes

40 nm-150 nm

Urine samples stored at -70°C, -20°C, or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of urine preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA, or proteins.

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

25 preps

Purification and RNA Isolation Kit