Streptococcus agalactiae TaqMan PCR Kit

FPD-007

Streptococcus agalactiae TaqMan PCR Kit

Streptococcus agalactiae TaqMan PCR kit is designed for the detection of Streptococcus agalactiae in milk.

Streptococcus agalactiae is a gram-positive bacterium belonging to group B streptococci. This bacteria is the most common type of pathogenic Streptococcus, and it is also the most harmful one, which can cause bovine mastitis. At the same time, the bacteria can cause sepsis in pregnant women and puerperium and meningitis in neonates, causing great harm.

Streptococcus agalactiae TaqMan PCR Kit is specially designed for the detection of Streptococcus agalactiae-specific DNA in foods such as milk in TaqMan technology-based real-time PCR. The detection of this kit is based on TaqMan PCR, which is simple, reliable and fast. Includes Master Mix, Primer & Probe Mix for target and PCR control assays, and positive and negative controls (nuclease-free water) to confirm kit reagent integrity.

Streptococcus agalactiae

Each Streptococcus agalactiae TaqMan PCR Kit is tested against predetermined specifications to ensure consistent product quality.

If the reagents are to be used only intermittently, they should be frozen in aliquots.

All reagents can be stored for 1 year at -20℃ without showing any reduction in performance.

This product should be stored at -20℃ upon arrival. Repeated thawing and freezing (> 2 ×) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay.

Follow general precautions. All specimens should be considered potentially infectious and handled accordingly.
1. Take protective measures.
2. Use sterile pipette tips.
3. Use aseptic technique.
4. Do not use consumables and equipment in dedicated areas for i) sample extraction, ii) reaction setup, and iii) amplification/detection.
5. Store and extract positive material (specimens, controls, and amplicons) separately from all other reagents.
6. Dispose of unused kit reagents and samples according to local, provincial or federal regulations.
7. The presence of PCR inhibitors may lead to false negative or invalid results. Potential mutations within the targeted region of the Streptococcus agalactiae genome covered by the primers in this kit may result in the undetectable presence of the pathogen.
8. Ensure that proper specimen collection, transport, storage, and handling techniques are followed to achieve optimum performance of this test.

24 rxn

MDx TaqMan 2× PCR Master Mix 350 µL
S. agalactiae Primer & Probe Mix 70 µL
S. agalactiae Positive Control 50 µL
Nuclease-Free Water (Negative control) 1.25 mL
Product Insert 1

Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
DNA Purification Kit
Disposable powder-free gloves
Benchtop microcentrifuge
Micropipettors
Sterile pipette tips with filters
PCR tubes
Vortex mixer
PCR reaction preparation station (Optional)

Milk

Ready to use format