Salmonella enterica TaqMan PCR Kit Dx

FPD-008

Salmonella enterica TaqMan PCR Kit Dx

Salmonella enterica TaqMan PCR kit Dx is designed for the detection of Salmonella enterica in milk.

Salmonella enterica has become an important foodborne pathogen that seriously threatens public health. S. enterica is a facultative anaerobic Gram-negative bacterium that can survive low temperature and freezing conditions. Most of the 1.3 billion human gastroenteritis cases caused by Salmonella each year are due to ingestion of contaminated foods such as raw or undercooked meat, seafood and eggs, and raw or unpasteurized milk and dairy products. Salmonella can also be contracted after eating fresh fruit or vegetables contaminated with infected fertilizers. Symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps, with the elderly, newborns, and immunocompromised individuals most susceptible.

Salmonella enterica TaqMan PCR Kit Dx is an in vitro diagnostic test designed to detect and quantify Salmonella enterica-specific DNA using real-time hybridization-fluorescence detection. The detection of this kit is based on TaqMan PCR, which is simple, reliable and fast. Includes Master Mix, Primer & Probe Mix for target and PCR control assays, and positive and negative controls (nuclease-free water) to confirm kit reagent integrity. Probes are labeled with fluorescent reporter dyes and quencher dyes. Probes specific to Salmonella enterica are labeled with the fluorophore FAM. Probes specific to PCR controls are labeled with the fluorophore HEX. DNA specific for Salmonella enterica can be detected in parallel using probes linked to distinguishable dyes.

Salmonella enterica

Each Salmonella enterica PCR Kit Dx is tested against predetermined specifications to ensure consistent product quality

If the reagents are to be used only intermittently, they should be frozen in aliquots.

Repeated thawing and freezing (> 3 ×) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay.
All reagents can be used until the expiration date specified on their labels.

All kit components should be stored at -20℃ upon arrival.

Follow general precautions. All specimens should be considered potentially infectious and handled accordingly.
1. Take protective measures.
2. Use sterile pipette tips.
3. Use aseptic technique.
4. Do not use consumables and equipment in dedicated areas for i) sample extraction, ii) reaction setup, and iii) amplification/detection.
5. Store and extract positive material (specimens, controls, and amplicons) separately from all other reagents.
6. Dispose of unused kit reagents and samples according to local, provincial or federal regulations.
7. The presence of PCR inhibitors may lead to false negative or invalid results. Potential mutations within the targeted region of the Salmonella enterica genome covered by the primers in this kit may result in the undetectable presence of the pathogen.
8. Ensure that proper specimen collection, transport, storage, and handling techniques are followed to achieve optimum performance of this test.

24 rxn

MDx TaqMan 2× PCR Master Mix Dx 550 µL
S. enterica Primer & Probe Mix Dx 2 × 70 µL
S. enterica Positive Control Dx-200,000
copies/μL 50 µL
Nuclease-Free Water 2 × 1.25 mL
Product Insert 1

Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
DNA Purification Kit
Disposable powder-free gloves
Benchtop microcentrifuge
Micropipettors
Sterile pipette tips with filters
PCR tubes
Vortex mixer
PCR tube centrifuge
PCR reaction preparation station (Optional)

Milk

Ready to use format