Product Name
Hoechst 33258
Product Overview
Hoechst 33258 is used for fluorescence imaging of cell nuclei. 33258 is provided as a 10 mg solid and a 10 mg/mL aqueous solution.
Description
The blue fluorescent Hoechst dye is a cell-permeable nucleic acid stain used in a variety of applications including sensitive detection (>3 ng) of DNA in the presence of RNA in agarose gels, automated determination of DNA, sensitive determination of cell number and Chromosome sorting. This product can be used in applications such as fluorescence microscopy, microplates, cuvettes, and flow cytometry.
Characteristic
Fluorescence excitation and emission profiles of Hoechst bound to dsDNA.
Applications
Fluorescence microscopy, microplate, cuvette, and flow cytometry.
Usage
Nuclear fluorescent probe
Stability
Stable under normal conditions.
Storage
-20℃, protect from light.
Introduction
Hoechst 33258 is a popular cell membrane-permeant nuclear counterstain. Hoechst 33258 emits blue fluorescence upon binding to the minor groove of dsDNA. Hoechst 33258 are widely used for nuclear counterstaining, apoptosis and cell cycle studies. Hoechst 33258 is provided as a 10 mg solid and a 10 mg/mL aqueous solution.
Handling
Always wear recommended Personal Protective Equipment. No special handing advice is required.
Category
Plant Organelle Fluorescent Probes
Excitation/Emission (nm)
352/461
Molecular Formula
C25H37Cl3N6O6
Chemical Name
Hoechst 33258
Appearance
yellow solution
Potential Health Hazard Effects
May cause eye irritation in susceptible persons.
May cause skin irritation in susceptible persons.
May be harmful by inhalation.
May be harmful if swallowed.
Polymerization
Hazardous polymerization does not occur.
Biodegradation
Does not bioaccumulate.
Notice
Use the reagent as soon as possible after unpacking!
Datasheet 
