Product Overview
The enzymes in this panel have been selected for their activity on a wideselection of substrates and in a widerange of pH, to encounter different synthetic needs of our customers.
Enantiopure alcohols can be obtained using our ADHs. (R) and (S)-enantiomers of acetophenone and its derivatives can be accessed by using these ADHs as well as racemic mixtures.
Description
This panel contains 6 alcohol dehydrogenase (ADH) enzymes for the reduction of ketones and aldehydes to the corresponding alcohols. The enzymes in this panel belong to different protein folds i.e., short chain ADHs, ketoreductases, both from prokaryotes and eukaryotes. Also included in the enzyme is metagenomic samples and engineered enzymes.
ADHs need a cofactor (NADH or NADPH) for reducing the carbonyl. To be able to use this cofactor in catalytic amounts, the oxidised cofactor has to be continuously reduced, this is called cofactor regeneration. The enzymes accept either or both NAD(P)H cofactors and their regeneration can be achieved by adding a cofactor regeneration enzyme (GDH or FDH, also provided in the kit) and its substrate (D-glucose or sodium formate, respectively). In some cases, the same ADH is able to regenerate the cofactor in presence of isopropanol.
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