Xylopine

ALK-0282

Xylopine

517-71-5

Xylopine is an aporphine alkaloid with cytotoxic activity on cancer cells. Xylopine induces oxidative stress, causes G2/M cell cycle arrest and apoptosis in cancer cells.

Xylopine

295.33

C18H17NO3

>98.0%

Solid

Plants >other families

4℃, sealed storage, away from moisture and light

Room temperature in continental US; may vary elsewhere.

COC1=CC=C2C3=C4C(CCN[C@]4([H])CC2=C1)=CC5=C3OCO5

Xylopine (3.5 μM-14 μM; 24-48 hours) displays potent cytotoxicity in a time- and does-depenpent manner. Xylopine (72 h) has cytotoxic activity, with IC50 values ranging from 6.4 to 26.6 μM in eight different cancer cell lines (MCF7, HCT116, HepG2, SCC-9, HSC-3, HL-60, K-562, and B16-F10). Xylopine (3.5 μM-14 μM; 24-48 hours) causes cell cycle block at the phase G2/M, which is followed by internucleosomal DNA fragmentation. Xylopine (3.5 μM-14 μM; 24-48 hours) significantly increases the early and late apoptosis, induces mitochondrial depolarization, and increases caspase-3 activation.Xylopine also causes an increase in the production of reactive oxygen/nitrogen species (ROS/RNS), including hydrogen peroxide and nitric oxide, but not superoxide anion, and reduces glutathione levels are decreased in Xylopine-treated HCT116 cells.HCT116 cells3.5 μM, 7 μM, and 14 μM24 hours, 48 hoursInduced G2/M phase arrest. HCT116 cells3.5 μM, 7 μM, and 14 μM24 hours, 48 hoursSignificantly increased the early and late apoptosis.