Pagrus major Gene Editing

Red seabream (Pagrus major) is a fish of the Sparidae family of Perciformes, native to the northwest Pacific Ocean, and lives mainly near coral reefs at a depth of 100 meters. Red seabream is popular in Japan because of its tender meat and rich nutrition, and it is a marine food fish with economic value.

Figure 1. Red seabream (Pagrus major) is small and has predominantly red scales with some blue spots.

Red sea bream has scales ranging from pink to purple-brown all over its body, and its brilliant scales are considered a symbol of good luck and good fortune in Japan; and appear in critical cultural festivals and weddings as valuable and luxurious food.

With the continuous development of sequencing technology, gene function research using red sea bream genome sequence can reveal the biodiversity and provide strong technical support to select and breed high-quality edible red sea bream species with strong disease resistance, tender meat, and short growth period.

Lifeasible is an expert in the fish gene editing industry with a mature and well-established gene editing technology platform based on the CRISPR/Cas9 system, guaranteeing you a high mutation rate Pagrus major gene editing service.

CRISPR/Cas9 Technology Services

CRISPR/Cas9 technology is an emerging gene-editing tool with high efficiency and precision that has now been demonstrated for gene editing in red sea bream. Our team of scientists used CRISPR/Cas9 technology to edit several genes involved in the metabolism and growth of red sea bream, including the muscle growth inhibitor gene (mstn).

We injected a mixture of Cas9 RNA and sgRNA-targeted RNA into artificially selected cultured red seabream fertilized eggs by microinjection, incubated them in seawater at a suitable temperature, and finally tested whether the mutation was successful by heterologous double-stranded mobility analysis (HMA). Ultimately, it was shown that red seabream with knocked-out growth suppressor genes obtained larger body size and muscle content and was able to shorten the breeding time.

Figure 2. Cloning of mstn and somatic mutagenesis analysis in embryos. (Kishimoto, et al. 2018)

Lifeasible has developed editing services for different genes of red seabream based on the genome sequence information already available. We can also provide various service options according to the needs of our clients and quickly analyze any problems encountered in the program's implementation. For more information or any inquiry request, please contact us.