Pisum sativum Transformation

Pisum sativum is the world's second largest edible legume crop, and it can be categorized into grain Pisum sativum, vegetable Pisum sativum, etc. With its nitrogen-fixing ability, Pisum sativum is an environmentally friendly crop, which has the ability to lose weight and increase efficiency, improve soil, etc., and plays a vital role in the sustainable development of agricultural systems. Transgenic technology is an essential means to study the function of plant genes and their mechanism of action, as well as one of the ways to improve traits, and the most widely used transgenic methods are mainly Agrobacterium-mediated methods, including Agrobacterium rhizogenes. Lifeasible, after years of development and technological research, independently developed a system of genetic transformation and cell regeneration for Pisum sativum, which significantly solved the problem of genetic transformation and cell regeneration in the field. Cell regeneration system significantly solves the problems faced in Pisum sativum transformation, such as long transformation cycle, high contamination rate, low transformation rate and so on.

What do we offer?

Transgenic technology is an essential means to study the function of plant genes and their mechanism of action, as well as one of the ways of trait improvement, and the most widely used transgenic methods are mainly Agrobacterium-mediated, including Agrobacterium rhizogenes-mediated and Agrobacterium hairy root-mediated methods. The transgenic transformation of Pisum sativum and most other legumes mediated by Agrobacterium rhizogenes has a long transformation cycle, high contamination rate, low transformation rate, and high difficulty in gene stabilization transformation, so we use Agrobacterium hairy root to induce the formation of hairy roots carrying the target genes to be widely used in legume crops. The general principle of this technology is to utilize Agrobacterium hairy root to induce the formation of transgenic hairy roots in the underground part of the plant, thus obtaining a combined seedling whose underground part is transformed and the aboveground part is non-transformed. In addition, we have established an efficient transgenic system for Pisum sativum, characterized by easy operation, low cost, high transformation efficiency, good reproducibility, short experimental period, and low contamination rate. In conclusion, in the study of the genetic transformation of Pisum sativum, we established the method of an efficient genetic transformation system mediated by Agrobacterium hairy root according to the problems in the genetic transformation of Pisum sativum, which can help researchers to save the cost of experiments further, shorten the experimental cycle, reduce the rate of contamination, and improve the convenience of experimental operation.

Our service content

• Cloning of target genes.
• Construction of transformation vector.
• Sterile seedling cultivation.
• Agrobacterium infestation.
• Induction of germination and rooting.
• Resistant T0 generation plants.
• Positive plant detection.

Benefits of genetic transformation of Pisum sativum

• Genetic Improvement. Agrobacterium rhizogenes genetic transformation can introduce specific genes into Pisum sativum that can improve or enhance its traits. Examples include improved disease resistance, tolerance, and increased yield.
• Improvement of product traits. Introducing specific genes into Pisum sativum improves product traits, e.g., improved appearance of pods, texture, storability, etc.
• Increase in nutritional value. Introducing DNA fragments carrying nutritional improvement genes can make Pisum sativum more nutritious.

Our service workflow

Why choose us?

• Experienced. Our expert team has rich service experience in Pisum sativum transformation; so far, we have completed several Pisum sativum genetic transformation services.
• Perfect service process. We provide a one-stop service for everything from customization of experimental processes to project progress and completion.
• Efficient plant transformation services. We are available year-round to conduct transformation projects, which saves our clients a lot of research time and improves project turnaround time.

Lifeasible is committed to plant functional gene research and agricultural biotechnology development. We specialize in plant genetic transformation systems and are also good at all kinds of molecular biology experimental techniques, which can provide professional and convenient technical support for researchers. If you are interested in us, please feel free to contact us.