Plant Chloroplast Genome Sequencing

Chloroplasts are essential organelles that convert light energy into chemical energy in plants and are semi-autonomous organelles with a unique genetic system. The genes involved in photosynthesis in chloroplasts are relatively conserved in size, structure, and content. The chloroplast genome is mainly used to encode proteins and ribosomal proteins that are closely related to photosynthesis. Analysis of the regulation of chloroplast gene expression and genetic characteristics can help advance the study of the origin and evolution of plant species and the relatedness between different species.

Lifeasible has a long history of molecular plant research and can provide chloroplast genome sequencing services for many species. We can provide personalized and optimized solutions to difficult chloroplast sequencing problems. We are committed to providing our clients with accurate chloroplast genome sequencing services to help botanists conduct effective research on plant phylogeny, population evolution, and genetics.

What We Offer

We take the most suitable sequencing solution according to the different needs of our clients.

• Sample collection: green tissue parts of plants, such as young leaves and leaves, are collected, and the samples are exposed to light for more than 6 h before processing.
• Extraction of total chloroplast DNA: total DNA from chloroplasts is extracted using kits, and the unique purification technique developed in the laboratory is used to reduce plant nuclear DNA contamination effectively.
• If we target chloroplast signature genes such as rbcL, matK, and trnH-psbA, we perform Sanger sequencing on the PCR products after gene amplification. Smaller fragments of Gap present in the next-generation of random sequencing are also sequenced again using Sanger sequencing for additional sequencing.
• For smaller chloroplast genomic analyses, we primarily provide Illumina random sequencing.
• For larger or more complex chloroplast genomic analyses, where neither sanger sequencing nor next-generation sequencing technologies are capable of complete sequencing, we can provide long-read PacBio long fragment sequencing to compensate for poor sequencing coverage.
• The raw data are de novo assembled under strict quality control to obtain a completed map of the chloroplast genome.
• Bioinformatics analysis: we offer analysis of coding genes, gene function annotation, comparative genomic analysis, etc. Personalized analyses, such as microsatellite sequence analysis and differential gene expression analysis, are also available.

Plant Species Covered by Our Service

Advantages

• Fast experimental cycle time
• Rigorous experimental system
• Optimized sequencing protocols
• Professional bioinformatic analysis

Applications

• Species classification
• Plastid genome cyclization
• Phylogenetic analysis, etc.

Our Service Process

Lifeasible has recruited a large number of experienced sequencing technicians to form a team of professional plant sequencing technicians who can provide affordable, fast, and accurate chloroplast sequencing solutions to our clients. If you have any technical difficulties with your chloroplast genome research, please feel free to contact us.