Protocols

Microspore Culture and Plant Regeneration of Brassica napus

In 1982, Lichter of Germany obtained haploid plants for the first time through free microspore culture of Brassica napus. Since the isolation technology of free microspores of Brassica napus is simple

Potato Stem Tip Detoxification

Potato (Solanum tuberosum) is an important crop in the world. It is an important dual-purpose crop for food and vegetable because of its short growth period, high yield, wide adaptability, rich nutrit

Strawberry Stem Tip Detoxification

Strawberry (Fragaria ananassa) is a perennial herb with high economic benefits. It mainly reproduces by stolons and is easily infected by one or more viruses during cultivation. At present, more than

Exfoliation and Culture of Plant Shoot Apical Meristems

Plant virus diseases are one of the important factors limiting agricultural production. After most vegetatively propagated plants are infected with viruses, their vegetative propagation characteristic

Creating Fragrant Corn with CRISPR/Cas9 Technology

Popcorn produces pyrroline substances through the Maillard reaction during the popping process, giving off a fragrant aroma. Studies have found that the loss of function of betaine aldehyde dehydrogen

Detection of Arabidopsis SNP Genetic Markers

Arabidopsis belongs to the Angiosperms, Dicots, Cruciferae, and is a biennial herb. SNP marker technology has unique advantages. It is more direct for comparing different genotypes, and therefore more

SSR Identification of Cotton Salt Tolerance

Cotton is the seed fiber of the Gossypium plant of the Malvaceae family. Breeding salt-tolerant cotton varieties will be a way to effectively develop and utilize saline-alkali land and promote sustain

AFLP Analysis of Genetic Diversity and Genetic Relationships of Spinach Germplasm

Spinach belongs to the genus Spinacia of the Amaranthaceae and subfamily Chenopodioideae and is an annual herbaceous plant. Randomly amplified DNA (RAPD) and other technologies have been reported in t

RAPD Analysis of Genetic Diversity for Salt Tolerance in Wheat

Wheat is an annual grass plant. It is restricted due to the scarcity of salt-tolerant germplasm resources. In particular, the salt-tolerance variation of wheat is very limited and the genetic basis is

Detection of Genetic Diversity of Rice Parents by RFLP

Rice is an annual grass plant. Whether it is conventional breeding or hybrid rice breeding, the selection of parents is very important. It requires the parents to carry the target traits or genes and