Protocols

Detection of Arabidopsis SNP Genetic Markers

Arabidopsis belongs to the Angiosperms, Dicots, Cruciferae, and is a biennial herb. SNP marker technology has unique advantages. It is more direct for comparing different genotypes, and therefore more

SSR Identification of Cotton Salt Tolerance

Cotton is the seed fiber of the Gossypium plant of the Malvaceae family. Breeding salt-tolerant cotton varieties will be a way to effectively develop and utilize saline-alkali land and promote sustain

AFLP Analysis of Genetic Diversity and Genetic Relationships of Spinach Germplasm

Spinach belongs to the genus Spinacia of the Amaranthaceae and subfamily Chenopodioideae and is an annual herbaceous plant. Randomly amplified DNA (RAPD) and other technologies have been reported in t

RAPD Analysis of Genetic Diversity for Salt Tolerance in Wheat

Wheat is an annual grass plant. It is restricted due to the scarcity of salt-tolerant germplasm resources. In particular, the salt-tolerance variation of wheat is very limited and the genetic basis is

Detection of Genetic Diversity of Rice Parents by RFLP

Rice is an annual grass plant. Whether it is conventional breeding or hybrid rice breeding, the selection of parents is very important. It requires the parents to carry the target traits or genes and

Separation of Proteins by Two-dimensional Gel Electrophoresis

Two-dimensional electrophoresis is a protein detection technology with higher resolution that combines isoelectric focusing electrophoresis and SDS-PAGE. The gel after two-dimensional electrophoresis

Determining The Relative Molecular Mass of Proteins Using SDS-PAGE

The determination of the relative molecular mass of proteins is of great significance in theory and practice. When a protein is electrophoresed in a polyacrylamide gel, its mobility depends on factors

Analysis of Wheat Peroxidase Isoenzymes by Vertical Polyacrylamide Gel Electrophoresis

In biology, isozymes can be used to study species evolution, genetic variation, cross-breeding, ontogeny, tissue differentiation, etc. Genetic variation in animals and plants can be identified by comp

Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis

Cellulose acetate membrane electrophoresis is an electrophoresis method that uses cellulose acetate membrane as a support. It is acetate of cellulose, produced by acetylation of the hydroxyl groups of

Agarose Gel Electrophoresis Detection of Plasmids

Agarose gel electrophoresis is an electrophoresis method that uses agar or agarose as a supporting medium. For samples with relatively large molecular weights, such as macromolecular nucleic acids, vi