Protocols

Separation of Proteins by Two-dimensional Gel Electrophoresis

Two-dimensional electrophoresis is a protein detection technology with higher resolution that combines isoelectric focusing electrophoresis and SDS-PAGE. The gel after two-dimensional electrophoresis

Determining The Relative Molecular Mass of Proteins Using SDS-PAGE

The determination of the relative molecular mass of proteins is of great significance in theory and practice. When a protein is electrophoresed in a polyacrylamide gel, its mobility depends on factors

Analysis of Wheat Peroxidase Isoenzymes by Vertical Polyacrylamide Gel Electrophoresis

In biology, isozymes can be used to study species evolution, genetic variation, cross-breeding, ontogeny, tissue differentiation, etc. Genetic variation in animals and plants can be identified by comp

Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis

Cellulose acetate membrane electrophoresis is an electrophoresis method that uses cellulose acetate membrane as a support. It is acetate of cellulose, produced by acetylation of the hydroxyl groups of

Agarose Gel Electrophoresis Detection of Plasmids

Agarose gel electrophoresis is an electrophoresis method that uses agar or agarose as a supporting medium. For samples with relatively large molecular weights, such as macromolecular nucleic acids, vi

Transformation of Tomato

Tomato is a plant of the Solanaceae family and is an annual or perennial herbaceous plant. Tomato is one of the most cultivated vegetable crops in the world. Because it has the advantages of self-poll

Transformation of Cucumber

Cucumber originates from the tropical rainforest areas at the southern foothills of the Himalayas. The botanical classification is Cucurbitaceae. Improving existing cucumber varieties through bioengin

Transformation of Arabidopsis thaliana

Principle Arabidopsis thaliana is a cruciferous plant, a biennial herb, 7-40 cm tall. It is widely used in research on plant genetics, developmental biology and molecular biology, and has become a typ

Pollen Tube Pathway-Mediated Cotton Transformation

In recent years, with the continuous improvement of transformation technology, the application of pollen tube pathway in agricultural molecular breeding has become more and more widespread. More than

Transformation of Immature Wheat Embryos Using Gene Gun Method

Principle Gene gun method is also called microprojectile bombardment, parti-cle bombardment and biolistics. The gunpowder-detonated gene gun was first developed by Sanford et al. (1987) of Conell Univ