Protocols

Extraction and Purification of Tobacco Leaf RNA

RNA is an intermediate product of gene expression and also the genetic material of RNA viruses. The manipulation of RNA plays an important role in molecular biology. Highly pure and complete RNA is ne

Extraction of Total Protein from Wheat Leaves

Protein is the material basis of life, the basic organic matter that constitutes cells, and the main carrier of life activities. Without protein there is no life. Therefore, it is a substance closely

Extraction and Purification of Rice Genomic DNA

DNA is the carrier of genetic information and the material basis of gene expression. Whether it is to study the structure and function of DNA or to conduct genetic engineering research, the DNA needs

Extraction and Purification of Plasmid DNA

Plasmid is the most commonly used carrier in molecular cloning, and the preparation of plasmid has become one of the most basic techniques in molecular cloning. The preparation of the plasmid DNA that

Production of Secondary Metabolites By Large-scale Plant Cell Culture

The traditional extraction and processing of plant active ingredients has the following deficiencies: limited by the distribution area of plants; affected by seasons, climate, disease and pest disaste

Selection and Storage of Plant Cell Lines

Screening is the basic method of plant cell selection and improvement. The plant cells obtained by various methods are often inhomogeneous and will show different characteristics (such as early resist

Measurement of Plant Cell Viability

In cell suspension culture, by measuring the viability of cultured cells and cell clusters, we can effectively understand the growth and viability of cells. Before protoplast culture, it is often nece

Regulation of Secondary Metabolite Synthesis in Plant Cell Culture

The growth of plant cells is often affected by various factors, which in turn affect the yield and quality of secondary metabolites produced by them. These factors include environmental factors and nu

Induction and Culture of Tobacco Callus

The induction and cultivation of plant callus are of great significance in both basic and applied research in plant science. Through this experiment, we can preliminarily master the aseptic technique

Content Analysis and Separation and Purification of Secondary Metabolites in Plant Culture

Many kinds of secondary metabolites will be produced during plant cultivation, and certain physical methods are required to separate and purify the metabolites we need for subsequent processing. There