Protocols

Selection and Storage of Plant Cell Lines

Screening is the basic method of plant cell selection and improvement. The plant cells obtained by various methods are often inhomogeneous and will show different characteristics (such as early resist

Measurement of Plant Cell Viability

In cell suspension culture, by measuring the viability of cultured cells and cell clusters, we can effectively understand the growth and viability of cells. Before protoplast culture, it is often nece

Regulation of Secondary Metabolite Synthesis in Plant Cell Culture

The growth of plant cells is often affected by various factors, which in turn affect the yield and quality of secondary metabolites produced by them. These factors include environmental factors and nu

Induction and Culture of Tobacco Callus

The induction and cultivation of plant callus are of great significance in both basic and applied research in plant science. Through this experiment, we can preliminarily master the aseptic technique

Content Analysis and Separation and Purification of Secondary Metabolites in Plant Culture

Many kinds of secondary metabolites will be produced during plant cultivation, and certain physical methods are required to separate and purify the metabolites we need for subsequent processing. There

Establishment of Plant Suspension Cell Lines

Cells in plant suspension culture have the characteristics of rapid growth, uniform metabolism, and the growth environment is easy to control. Because the cultured cells are sensitive to various exter

Micropropagation Technique of Banana

The biotechnology applied in banana production is mainly to provide seedlings of superior varieties through tissue culture. At present, almost all banana cultivars with commercial value are triploid,

Micropropagation Technique of Phalaenopsis amabilis

Phalaenopsis amabilis is a tropical epiphytic orchid belonging to the genus Phalaenopsis of the family Orchidaceae, known as the "Queen of Orchids". Its flowers are as big as butterflies, be

Tobacco Micropropagation Technology

Tobacco (Nicotiana tabacum) belongs to the Solanaceae plant, is an important industrial raw material, and is also an important one of the four typical experimental plants (tobacco, petunia, carrot, an

Apple Endosperm Culture Technology

Apple is the main tree species cultivated in deciduous fruit trees, and it is also one of the tree species with the widest cultivated area and high yield in the world. Endosperm of angiosperm is one o