Protocols

Establishment of Plant Suspension Cell Lines

Cells in plant suspension culture have the characteristics of rapid growth, uniform metabolism, and the growth environment is easy to control. Because the cultured cells are sensitive to various exter

Micropropagation Technique of Banana

The biotechnology applied in banana production is mainly to provide seedlings of superior varieties through tissue culture. At present, almost all banana cultivars with commercial value are triploid,

Micropropagation Technique of Phalaenopsis amabilis

Phalaenopsis amabilis is a tropical epiphytic orchid belonging to the genus Phalaenopsis of the family Orchidaceae, known as the "Queen of Orchids". Its flowers are as big as butterflies, be

Tobacco Micropropagation Technology

Tobacco (Nicotiana tabacum) belongs to the Solanaceae plant, is an important industrial raw material, and is also an important one of the four typical experimental plants (tobacco, petunia, carrot, an

Apple Endosperm Culture Technology

Apple is the main tree species cultivated in deciduous fruit trees, and it is also one of the tree species with the widest cultivated area and high yield in the world. Endosperm of angiosperm is one o

Wheat Embryo Culture Technology

Wheat is one of the important food crops, and embryo culture of wheat can not only be used for plant regeneration, but also can break seed dormancy through immature embryo culture to induce dormant se

Chrysanthemum Flower Organ Culture Technology

Chrysanthemum is one of the four largest cut flowers in the world. The high regenerative capacity of floral organ cells makes it a good material for studying cell morphogenesis and also for the study

Tomato Isolated Root Culture Technology

Root culture is mostly used to study root physiological metabolism, organ differentiation and morphogenesis. Root cell cultures can also be subjected to mutagenesis to screen out mutants for plant bre

Media Preparation and Sterilization

Using mother liquor to prepare medium can not only improve work efficiency, but also reduce weighing errors of substances with very small amounts. The medium contains a lot of organic matter, especial

Preparation of Medium Mother Liquor

Before preparing the culture medium according to the culture medium formula, for the convenience of use and accurate dosage, the the macroelements, trace elements, iron salts, organic matter, and horm