Recombinant Proteins Expression in Plants

Recombinant protein plant expression systems offer cost and safety advantages and have good potential for application in developing high value-added recombinant protein platforms. Plant protein expression systems mainly include transgenic plants, chloroplast-transformed plants, transient expression systems, and cell suspension cultures.

Lifeasible is dedicated to researching recombinant plasma white expression and preparation in plants. We have rich experience in recombinant protein plant expression, and we can provide customers with fast and precise customized services to meet their project needs.

Advantages of Recombinant Protein Expression in Plants

• Low upstream production costs.
• Easy gene conversion, high success rate.
• Short cycle time, high expression amount, easy to purify.
• Stable traits of transgenic plants, easy to accumulate multiple genes.
• Low contamination by animal pathogens.
• Plant expression system is more suitable for mass production.

Our Service for Recombinant Proteins Expression in Plants

Plant expression of recombinant proteins, as a platform for the production of high-value-added recombinant proteins, has significant advantages in terms of cost and safety, and plants have become a highly promising protein expression system recognized by researchers after microbial and mammalian expression systems. Currently, we provide plant expression systems, including transgenic plants, chloroplast transformation plants, transient expression systems, and cell suspension cultures. We provide the following services for recombinant protein plant expression.

Acquisition and synthesis of target genes

We synthesize the target gene sequences required for customer research according to customer requirements. PCR amplification, synthesis, or enzymatic digestion can obtain these gene sequences.

Gene cloning and vector construction

We clone the target gene into a plant expression vector. This vector contains promoters, terminators, selection tags, and other regulatory elements that control protein expression for efficient expression of the target protein.

Plant tissue culture

Plant tissue culture technology is utilized to grow transgenic plants or cells. We will optimize the culture medium and growth conditions according to the characteristics of different plants to promote the growth of transformed cells and the accumulation of target proteins. Successfully transformed plants are subsequently identified by antibiotic screening or other labeling methods.

Transient or stable protein expression

Choosing the appropriate expression system is intuitively important for recombinant protein plant expression services; for example, we can obtain transgenic plants capable of long-term expression of target proteins through Agrobacterium-mediated transient transfection or stable transformation. We need to combine the characteristics of the target protein and the experimental requirements to make a comprehensive analysis in selecting the expression system.

Protein purification

The target protein is extracted and purified from the transgenic plant or cell culture. This step requires specific purification techniques, such as affinity chromatography and ion exchange chromatography, to purify the target protein and ensure that a highly pure recombinant protein is obtained. When we choose purification techniques, we base the choice of purification method on the nature of the target protein, and sometimes, we can also choose and use a combination of techniques based on the characteristics of the target protein to obtain high-purity recombinant proteins.

Protein detection and analysis

Purified recombinant proteins are subjected to quality testing, including determining purity, activity, concentration, and other parameters, to ensure that their quality meets the requirements. In addition, we evaluate their biological activity, stability, and other tests.

Fig.2 Our service process. (Lifeasible)

Why Choose Us?

• Experienced. We have rich experience in recombinant protein plant expression and can provide customers with full process services from plant tissue culture to protein production.
• High-quality. Our recombinant protein plant expression services are efficient, safe, cost-effective, and very suitable for scientific research.
• One-stop. We have set up several R&D departments, including molecular cell biology, protein production and antibody preparation research teams, to provide one-stop service for recombinant protein plant expression.
• Innovation. We follow the cutting-edge of recombinant protein plant expression and constantly upgrade our technical strength and R&D platform with the spirit of innovation.

Lifeasible provides industry-leading recombinant protein plant expression. We conduct our R&D services with a strict quality control system to ensure reliable and advanced customer service support. If you are interested, please feel free to contact us.