Gene Editing Services in Chloroplasts of Model Plants

Plants and algal depend on photosynthesis that happens inside chloroplasts to manufacture the complex organic molecules they require as a source of energy. Model plants have been widely studied and used in plant biology, due to the features of relatively small genome and short lifecycle, as well as they are highly homologous with other complex plants.

For assisting researchers making greater breakthrough, Lifeasible provides gene editing services in chloroplasts of model plants.

Arabidopsis thaliana gardening

Overview of Model Plants

One of the best-known model plants is Arabidopsis thaliana, also called thale cress, mouse-ear cress or Arabidopsis. With relatively short lifecycle (can be completed in six weeks), A. thaliana is widely used in plant biology and genetics. It was the first plant to have its genome sequenced, since then, it has been widely used as a model plant in molecular biology.

A. thaliana is widely used in the study of plant physiology, such as the genetic basis of photosynthesis, flowering and metabolism. Besides, many model systems have been developed to understand the subdiscipline of plant pathology, which is the interaction between plants and pathogens causing disease.

The genome of chloroplast in A. thaliana is a circular DNA composed of 154,478 bp with a total of 87 potential protein-coding genes, which is a size typically encountered in most flowering plants. Meanwhile, the translated proteins from protein-coding genes are highly similar to those in Nicotiana tabacum and other plants. Therefore, studies in model plants can make great contribution to reveal the functional genomics in higher plants.

Our Services

Lifeasible provides gene editing services in chloroplast for assisting researchers studying mechanism of all vital activities in chloroplast, including gene knock-in and gene knock-out services. Our experienced scientists dedicate to provide trustable and satisfying services for our customers.

Lifeasible offers gene editing services in model plants including but not limited to follows.

Species Introduction Features
Arabidopsis thaliana Also called the thale cress, mouse-ear cress or arabidopsis. It's the most popular model plants. Small genome size; short life cycle
Nicotiana tabacum Known as tobacco, is the model plant of plant-pathogen studies. As well as chloroplast genomic studies. Small genome size
Oryza sativa Commonly known as rice. It's the model plant of cereal biology. Small genome size
Populus trichocarpa Known as cottonwood. It's model plant in forest genetics and woody plant studies. Small genome size; fast grow
Selaginella moellendorffii Remnant of an ancient lineage of vascular plants, it's key to understand the evolution of land plants. Small genome size
Brachypodium distachyon Commonly called purple false brome or stiff brome. It's model for temperate cereals. Small genome size; short life cycle; efficient transformation system
Setaria viridis Known as green foxtail, green bristlegrass and wild foxtail millet. It's model grass for C4 photosynthesis. Small genome size; C4 photosynthesis
Lotus japonicus Model plant od legume that is used to study nitrogen fixation. Small genome size; short life cycle
Lemna gibba Smallest flowering plant with a common name "duckweed". Small genome size; fast grow
Zea mays Known as corn and maize. It's model plant of cereal. Large chromosome pairs; large number of transposons (jumping genes)

Gene Knock-out Services in Chloroplasts of Model Plants

  • Single Gene Knock-out Services
    In order to assist our global customers understanding the regulatory mechanism of reactions happen in chloroplasts of model plants, Lifeasible provide site-specific gene knock-out services.
  • Markerless Multiple Gene Knock-out Services
    Lifeasible provides our customers multiple gene knockout services for studying gene-gene interaction and redundant gene function. Our scientists established a robust toolkit of multiple site-specific recombinases for implementing markerless multiple gene knock-out.

Gene Knock-in Services in Chloroplasts of Model Plants

We offer gene knock-in service in chloroplast for customers who need overexpressed or knocked-in chloroplasts of model plants for further research.

Information Needed from Customers
  • Information of yeast strain.
  • Information of target gene.
Delivery Results
  • Experimental report, including materials, methods, parameters, results, quality control report.
  • Strain of interest
Project Cycle
  • Single gene knock-out/knock-in: 4-6 weeks.
  • Multiple gene knock-out/knock-in: According to the number of target genes.

Service Process

Service process

Advantages

Innovative

Innovative

Customer-tailored

Customer-tailored

Efficient

Efficient

High-quality

High-quality

Lifeasible offers our customers innovative, efficient, customer-tailored and high-quality gene editing services. And we promise to deliver the results and products on time and with high quality. We serve researchers all over the world with heart and soul. Please feel free to contact us.

For research use only. Not intended for any clinical use.
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