Gene Editing Services in Pichia

Yeast has been contributing to human society for thousands of years. In addition to their applications in food industry, they have been used as model organisms in biology research. Pichia is a genus of yeast of potential application in both industrial production and biology research.

Lifeasible offers Pichia genome editing services based on recombinases system, with which, our global customers can launch gene functional studies and generate new strains of interest.

Overview of Pichia

Pichia is a genus of yeast in family Pichiaceae. Yeasts of Pichia are heterotrophs, which means they can use several carbon sources for living, including glucose, glycerol and methanol.

• Ethanol Fermentation
  Some of them interfere the process of fermenting ethanol, while some of them can combat Aspergillus flavus, which produces aflatoxins.
• Expression System
  They are also widely used in molecular biology and biotechnology as an expression system. Pichia yeasts have been genetically modified to produce renominate proteins and various metabolic compounds.
• Host of Producing Proteins
  Due to its ability to grow to high cell densities and producing high titers of heterologous protein, it becomes one of the most popular hosts of producing heterologous proteins, including industrial enzymes.

Typical Strains of Pichia

• Pichia pastoris
  P. pastoris uses methanol as source of carbon and energy. It plays important role in biochemical research and biotechnical industry. P. pastoris serves as an expression system of protein producing, as well as being model organism for genetic research.
• Pichia methanolica
  P. methanolica can grow in methanol media. It has been used as cell factory for producing pharmaceutical proteins, like insulin, hepatitis vaccines and other medical compounds.
• Pichia anomala
  P. anomala is used as preventive, also called biocontrol agent, for undesired fungi or mold. And it's also utilized in wind producing, grain storage, fruit storage and many other applications.

Our Services

Lifeasible provides gene editing services based on recombinases system in Pichia. Our services include gene knock-in and gene knock-out in yeasts of Pichia. With our mature yeast gene editing technology, customers can explore further applications of yeasts of Pichia genus.

Gene Knock-out Services in Pichia

• Markerless Single Gene Knock-out Services

In order to assist our global customers understanding the regulatory mechanism of reactions happen in Pichia, Lifeasible provide site-specific gene knock-out services.

• Markerless Multiple Gene Knock-out Services

Lifeasible provides our customers multiple gene knockout services for studying gene-gene interaction and redundant gene function. Our scientists established a robust toolkit of multiple site-specific recombinases for implementing markerless multiple gene knock-out in Pichia.

Gene Knock-in Services in Pichia

We offer gene knock-in service in Pichia for customers who need overexpressed or knocked-in yeast strains for further research.

Service Process

Advantages

Lifeasible offers our customers innovative, efficient, customer-tailored and high-quality gene editing services. And we promise to deliver the results and products on time and with high quality. We serve researchers all over the world with heart and soul. Please feel free to contact us.