Catalog Number: RNPP-74353896
| Size: 200µl 2ml Customization | Inquiry |
|---|
| Gene ID: | PP7435_Chr3-0980 |
| Gene Name: | TOP1 |
| Gene Location: | chr3: 1756147..1756345 |
| DNA Length (bp): | 199 |
| Protein Length (aa): | 65 |
| Gene Description: |
DNA topoisomerase I (EC 5.6.2.1) (DNA topoisomerase 1) Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone |
| Medium: |
YPD + 1M Sorbitol broth 30% glycerol |
| Shipping condition: | Frozen Gel Packs |
| Storage condition/Stability: |
-80℃/At least 12 months 4°C/Up to 1 week |
| Hazardous: | No |
Disclaimer
This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.
