Catalog Number: RNPP-74352318
| Size: 200µl 2ml Customization | Inquiry |
|---|
| Gene ID: | PP7435_Chr2-0725 |
| Gene Name: | UBA4 |
| Gene Location: | chr2: 1347232..1348521 |
| DNA Length (bp): | 1290 |
| Protein Length (aa): | 429 |
| Gene Description: |
Adenylyltransferase and sulfurtransferase UBA4 (Ubiquitin-like protein activator 4) [Includes: Adenylyltransferase UBA4 (EC 2.7.7.-); Sulfurtransferase UBA4 (EC 2.8.1.-)] Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln) Acts by mediating the C-terminal thiocarboxylation of sulfur carrier URM1 Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 Subsequently, a transient disulfide bond is formed Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation May also be involved in protein urmylation |
| Medium: |
YPD + 1M Sorbitol broth 30% glycerol |
| Shipping condition: | Frozen Gel Packs |
| Storage condition/Stability: |
-80℃/At least 12 months 4°C/Up to 1 week |
| Hazardous: | No |
Disclaimer
This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.
