Rhododendron simsii, also known as azalea, is a famous flowering plant with high ornamental value and cultivated in parks worldwide. In addition to its ornamental value, azalea also has medicinal value. Azalea can be used as a medicine for the whole plant, which can be used to treat internal injuries, cough, kidney deficiency, and menstrual disorders. Rhododendron is hardy (down to -39.4°C), and it prefers acidic soil. Therefore soil scientists often use azalea as an indicator crop for acidic soils. However, azalea's preference for acids and salt avoidance is the biggest limiting factor for cultivation and utilization. As higher demands were placed on azalea cultivation, genetically modified azaleas came into being. In recent years, research on genetic modification of azaleas has been developing, including changing leaf color, improving disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, etc.
Lifeasible provides a one-stop azalea genetic transformation service, covering the whole process of transgenic project consultation, experimental design, vector construction, plasmid transformation, positive seedling screening, PCR identification, and post-sale seedling transplanting guidance. Our versatile transformation platform is based on an efficient, stable, and mainstream Agrobacterium infestation method, providing a wide range of Agrobacterium strains, including C58, LBA4404, EHA105, GV2260, GV3101, supporting different types of resistance screening. To meet the various transformation needs of our customers, we also offer commercial and custom vectors with variant selection markers such as kanamycin, thaumycin, glufosinate, and G418. We have a variety of genetic engineering technologies for you to choose from for vector and plasmid transformation as follows.
Customers can provide constructed azalea transformation vectors directly, and Lifeasible also offers vector construction services.
| Concentration | Volume | Other requirements |
| 80-100 ng/μL | ≥10 μL | no degradation & no contamination |
| Bacterial broth | Bacterial plate |
| Bacterial broth up to 1-year-old, preserved in glycerol | Bacterial plate activated within one week |
*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.
*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.
*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.
Experienced experts are arranged throughout the entire process of azalea gene transformation to provide you with pre-project consultation, experiment customization, vector construction, professional operation, and progress reports. Experiment progress is updated regularly, and we also keep our clients informed when the genetic transformation experiment reaches a critical point. You can also contact the staff at any time to follow up on the progress of the experiment. The experiment's success will be based on the positive identification of the target gene. We will also take care of any after-sales needs to the end.
Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in rhododendron simsii transformation. We can provide customized solutions to help you study a wide range of azalea varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
