Rhododendron Simsii Transformation

Rhododendron simsii, also known as azalea, is a famous flowering plant with high ornamental value and cultivated in parks worldwide. In addition to its ornamental value, azalea also has medicinal value. Azalea can be used as a medicine for the whole plant, which can be used to treat internal injuries, cough, kidney deficiency, and menstrual disorders. Rhododendron is hardy (down to -39.4°C), and it prefers acidic soil. Therefore soil scientists often use azalea as an indicator crop for acidic soils. However, azalea's preference for acids and salt avoidance is the biggest limiting factor for cultivation and utilization. As higher demands were placed on azalea cultivation, genetically modified azaleas came into being. In recent years, research on genetic modification of azaleas has been developing, including changing leaf color, improving disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, etc.

What We Offer

Lifeasible provides a one-stop azalea genetic transformation service, covering the whole process of transgenic project consultation, experimental design, vector construction, plasmid transformation, positive seedling screening, PCR identification, and post-sale seedling transplanting guidance. Our versatile transformation platform is based on an efficient, stable, and mainstream Agrobacterium infestation method, providing a wide range of Agrobacterium strains, including C58, LBA4404, EHA105, GV2260, GV3101, supporting different types of resistance screening. To meet the various transformation needs of our customers, we also offer commercial and custom vectors with variant selection markers such as kanamycin, thaumycin, glufosinate, and G418. We have a variety of genetic engineering technologies for you to choose from for vector and plasmid transformation as follows.

Service Flow

1. Sample submission requirements

Customers can provide constructed azalea transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Experienced experts are arranged throughout the entire process of azalea gene transformation to provide you with pre-project consultation, experiment customization, vector construction, professional operation, and progress reports. Experiment progress is updated regularly, and we also keep our clients informed when the genetic transformation experiment reaches a critical point. You can also contact the staff at any time to follow up on the progress of the experiment. The experiment's success will be based on the positive identification of the target gene. We will also take care of any after-sales needs to the end.

3. Rhododendron simsii transformation results in delivery

• First, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• Based on our versatile azalea genetic transformation platform, we are able to provide stable and rapid transgenic technology services for a wide range of azalea species.
• We have a professional technical team with many years of experience in azalea genetic transformation, which can guarantee high efficiency and success rate of experiments to a certain extent.
• We use the mainstream Agrobacterium transformation method, which is efficient, stable, and based on single copy insertion and supports a variety of screening for fungal resistance, such as Chaumycin, G418, Basta, etc.
• When you don't have time to manage breeding work or can't breed in special seasons, our azalea transgenic service is the best choice for you.

Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in rhododendron simsii transformation. We can provide customized solutions to help you study a wide range of azalea varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.