Setaria viridis Transformation

Setaria viridis is an annual herbaceous plant named for its resemblance to a dog's tail. It is native to temperate and warm temperate regions of Eurasia and is now widely distributed in the world's temperate, warm temperate, and tropical regions. Setaria viridis has medicinal value and certain ornamental value. Setaria viridis has medicinal and ornamental values and can be used as a wild vegetable and fodder, with certain food and feeding value.

Lifeasible is committed to providing technical services for plant gene function and industrial application. After years of development and technological research, we provide reliable Setaria viridis genetic transformation technology services, which help researchers significantly improve the transformation efficiency of Setaria viridis, shorten the transformation cycle, and provide a new technology platform for Setaria viridis transformation research.

What do we offer?

• Callus selection and induction. We will select embryonic callus at different stages of specific strains of Setaria viridis as explants to promote callus formation through efficient embryonic callus induction techniques.
• Agrobacterium strain selection. Agrobacterium strains suitable for transformation were selected, and culture conditions were optimized to improve transformation efficiency.
• Immersion and co-culture. We imbibed the healing tissues in a suspension containing Agrobacterium, and promoted the interaction between Agrobacterium and the cells of the healing tissues through the co-culture method.
• Resistant callus screening. This step involves using specific screening conditions, such as antibiotics, to screen out calluses containing transformed DNA and eliminate untransformed tissues.
• Callus seedling formation. The screened resistant callus is transferred to a suitable medium to promote their differentiation into complete plants.
• Optimization of transformation efficiency. We further improved the transformation efficiency by adjusting a series of conditions, such as seed conditions and the composition of the healing tissue induction medium.
• Molecular validation. We validate the transformation events by molecular biology methods, such as PCR and Southern blot, to ensure that the exogenous genes have been integrated into the Setaria viridis genome.

Our experimental procedure

Our research objectives of Setaria viridis transformation

• C4 photosynthesis research. Setaria viridis is a model plant for C4 photosynthesis, and its genetic transformation research helps to deeply understand the molecular mechanism of C4 photosynthesis.C4 plants have high efficiency of light energy utilization, and the study of its genetic mechanism is of great significance to improving photosynthesis in C3 crops.
• Establishment of model plants. Setaria viridis has characteristics such as rapid life cycle, small genome, and ease of growth, making it an ideal model for monocotyledon research. The development of genetic transformation technology is the key to studying Setaria viridis as a model plant.
• Gene function studies. Through genetic transformation technology, gene function can be verified in Setaria viridis, to identify genes related to growth and development, response to adversity and other biological processes.
• Crop breeding research. Setaria viridis is related to many important food crops, and the results of its genetic transformation research can help to improve the traits of these crops, such as increasing yield, improving quality, and enhancing adaptability to adversity.

Why choose us?

• Well-established technology platform. We have established and developed an advanced and reliable genetic transformation technology system based on years of research experience.
• High experimental success rate. Our experienced team of experts and advanced technology platforms can guarantee the success rate of dogwood transformation.
• Efficient experimental process. We constantly optimize the genetic transformation system and can carry out genetic transformation projects at any time to ensure that the dogwood genetic transformation project is fast, efficient and stable.
• Perfect service support. Our service covers the whole process of customer project development, thus helping customers save a lot of time and energy.

Lifeasible has leading gene editing tools and an experienced team of researchers in developing plant genetic transformation technologies. We aim to help researchers break through the challenges of genetic transformation and advance the study of plant gene function and gene editing breeding. If you are interested in us, please feel free to contact us.