Targeted Metabolomics of Plant Hormone

Plant hormones are active organic substances synthesized in plants, which can be transported from the site of production to the site of action. They can produce a certain physiological effect on plants in trace concentrations and play an essential role in regulating gene expression, growth, development, and response to the environment in plants. Plant hormones mainly include growth hormones (AUXs), gibberellins (GAs), cytokinins (CTKs), abscisic acid (ABA), and ethylene (Eth). In addition, it has been found that jasmonic acid, salicylic acid, polyamines, and oleoresinolide are involved in a variety of physiological processes in plant growth, including defense responses, and play an active role in pathogen resistance. Thus they can be regarded as a new class of plant hormones.

Lifeasible provides phytohormone-targeted assays for the absolute quantification of phytohormones to provide theoretical and technical support for plant development, molecular genetics, and cell biology research. We collect metabolomic data of biological samples through MRM mode to achieve absolute characterization of metabolites. We combine the external standard quantification method with the standard standard curve to realize the absolute quantification of metabolites in the samples.

We provide a database of plant hormones

Applied directions

• Plant growth and development research.
• Research on biotic and abiotic plant stress interactions.
• Functional analysis of plant genes and metabolic pathways.
• Crop quality traits and breeding improvement.
• Fruit and vegetable storage research.

Advantages of our technology

• Advanced platform. Using high precision and high-resolution high-end precision target detection mass spectrometer to realize precise target identification.
• Self-built database. We have established a database of standard products for 8 plant hormones, which can realize absolute qualitative and quantitative and provide 8 standard curves for 8 substances.
• Precise quantification. We adopt the isotope internal standard method for absolute quantification, and the quantitative structure is accurate and reliable.
• Wide coverage. Our assay covers growth hormone, cytokinin, gibberellin, abscisic acid, ethylene, oleuropein sterols, methyl jasmonate, salicylic acid, and unicarpal lactone.
• Biofid analysis. We cover other differential analyses, PCA, OPLS-DA, analysis of variance, etc., in addition to the usual qualitative quantitative analyses.
• Manual proofreading. A rigorous manual proofreading process ensures the accuracy and reliability of the method.

Sample requirements

• Plant roots / stems / leaves / fruits / seeds.
• More than 1 g wet weight/sample.
• Liquid nitrogen quickly freezes and store at -80 °C.
• Send on dry ice.

Our technology routes

Lifeasible is an industry leader in the field of plant metabolism and aims to provide high-quality testing services to our clients based on our years of service experience and advanced technology platform. If you are interested in us, please feel free tocontact us.