Torreya grandi Transformation

Torreya grandis is an evergreen tree with significant economic, social, and ecological values. Torreya grandis is mainly distributed in the central subtropics, its trunk is tall, upright, beautiful, and evergreen, has strong resistance to soot and is seldom infested with pests and diseases, it is very rich in ornamental value, and it is excellent tree species for gardening and yard greening. However, due to the long biological cycle of Torreya grandis, the traits are easily affected by the environment and other factors, which limits the development of its breeding work. Currently, there are few studies on the use of genetic engineering to improve the breeding of Torreya grandis. Therefore, the genetic improvement of Torreya grandis using genetic engineering to increase crop yield, improve quality, and enhance resistance has received attention from researchers.

Lifeasible is dedicated to the plant field, and we aim to provide researchers with efficient and reliable plant genetic transformation services using advanced biotechnology. We use the Agrobacterium-mediated method to realize the genetic transformation of Torreya grandis. Customers can provide target genes, plasmids, Agrobacterium, etc so that we can complete the whole experimental process, from constructing vectors to obtaining transgenic seedlings for you.

What do we offer?

We conducted a study on the genetic transformation of Torreya grandis using Agrobacterium-mediated genetic transformation using Torreya grandis seed young embryos as recipient material. We used Torreya grandis seed young embryos as transgenic recipient materials. We established a reliable and efficient Agrobacterium-mediated genetic transformation of Torreya grandis by comparing the effects of recipient embryo age, Agrobacterium sap concentration, infestation time, and antibiotic mass concentration on the efficiency of genetic transformation, and by positively screening genetically transformed Torreya grandis young embryo cultures. Genetic transformation system.

Our services of Torreya grandis transformation

Optimization of conditions for genetic transformation of Torreya grandis embryos

• Screening for optimal embryonic age of young Torreya grandis embryos.
• Screening for optimal absorbance of Agrobacterium infestation.
• Screening for optimal time of Agrobacterium infestation.

Screening of Agrobacterium-mediated genetic transformation and resistance of young Torreya grandis embryos

• After selecting well-grown Torreya grandis embryos in the optimal concentration of Agrobacterium infestation solution and incubating in suspension for the optimal infestation time, the embryos were transferred to a specific solid medium for co-culture, and the resistant embryos were obtained.

Positive fluorescent signal detection and PCR identification of green fluorescent protein gene

• It used to identify the young embryo cultures of Torreya grandis as successful in transformation.
• We provide Agrobacterium-mediated genetic transformation of Torreya grandis, which has the advantages of simple operation, low copy number, high transformation efficiency, good reproducibility, and low incidence of gene silencing. The Torreya grandis genetic transformation service we provide is significant for Torreya grandis genetic engineering research, promoting Torreya grandis germplasm innovation and industrial development.

Our service workflow

Notice to customers

• Customers can directly provide the constructed Torreya grandis transformation vector or entrust us to carry out the related vector construction service.
• If the customer needs to do a special species, it will increase the experiment time and the need to provide the corresponding species seeds or seedlings.
• We provide independent T0 generation seedlings by default, and the positive result of PCR identification of resistance genes will be taken as the closing standard. For gene-editing seedlings, we provide positive T0 generation seedlings with specific mutation information.

Advantages of our technology

• Experienced. We have a professional technical team engaged in plant genetic transformation for many years, which can guarantee the success rate of the experiment.
• Efficient experimental process. We can carry out genetic transformation projects at any time and adopt specific transformation methods for particular plant species to help customers save transformation time.
• Mature genetic transformation system. We can ensure that any abnormalities in the experiment can be detected and communicated to the customer promptly and that the abnormalities can be resolved in time to ensure that the experiment proceeds smoothly.

Lifeasible provides professional, reliable, and one-stop technical support and research services for plant genetic transformation for research institutions and seed companies. We have a lot of experience in gene editing, vector construction, plant transgenesis, and other fields. If you are interested in us, please feel free to contact us.