Torreya grandis is an evergreen tree with significant economic, social, and ecological values. Torreya grandis is mainly distributed in the central subtropics, its trunk is tall, upright, beautiful, and evergreen, has strong resistance to soot and is seldom infested with pests and diseases, it is very rich in ornamental value, and it is excellent tree species for gardening and yard greening. However, due to the long biological cycle of Torreya grandis, the traits are easily affected by the environment and other factors, which limits the development of its breeding work. Currently, there are few studies on the use of genetic engineering to improve the breeding of Torreya grandis. Therefore, the genetic improvement of Torreya grandis using genetic engineering to increase crop yield, improve quality, and enhance resistance has received attention from researchers.
Lifeasible is dedicated to the plant field, and we aim to provide researchers with efficient and reliable plant genetic transformation services using advanced biotechnology. We use the Agrobacterium-mediated method to realize the genetic transformation of Torreya grandis. Customers can provide target genes, plasmids, Agrobacterium, etc so that we can complete the whole experimental process, from constructing vectors to obtaining transgenic seedlings for you.
We conducted a study on the genetic transformation of Torreya grandis using Agrobacterium-mediated genetic transformation using Torreya grandis seed young embryos as recipient material. We used Torreya grandis seed young embryos as transgenic recipient materials. We established a reliable and efficient Agrobacterium-mediated genetic transformation of Torreya grandis by comparing the effects of recipient embryo age, Agrobacterium sap concentration, infestation time, and antibiotic mass concentration on the efficiency of genetic transformation, and by positively screening genetically transformed Torreya grandis young embryo cultures. Genetic transformation system.
Optimization of conditions for genetic transformation of Torreya grandis embryos
Screening of Agrobacterium-mediated genetic transformation and resistance of young Torreya grandis embryos
Positive fluorescent signal detection and PCR identification of green fluorescent protein gene
Lifeasible provides professional, reliable, and one-stop technical support and research services for plant genetic transformation for research institutions and seed companies. We have a lot of experience in gene editing, vector construction, plant transgenesis, and other fields. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following: