Zoysia japonica is mainly distributed in eastern Asia but also in Africa and other warm regions. Zoysia japonica grows on hillsides, plains, and seashore grasslands. It is a widely used warm-season turfgrass with many excellent characteristics. Zoysia japonica is characterized by salt resistance, drought resistance, and few pests and diseases, and it is widely used in home lawns, golf courses, sports fields, park lawns, etc. Therefore, Zoysia japonica has an essential value in of developing and utilizing lawn grasses. Therefore, Zoysia japonica has an essential value in developing and utilizing turfgrass. However, Zoysia japonica is not cold-tolerant, and its ornamental properties will be seriously reduced when the temperature is lower than 10℃. Therefore, it is of great significance to utilize biotechnological means to introduce relevant resistance genes to enhance the resistance of Zoysia japonica and to extend its green period.
Lifeasible has established a sound and efficient genetic transformation platform for Zoysia japonica after years of research based on the problems in the genetic transformation of Zoysia japonica. The Zoysia japonica genetic transformation service we provide can help researchers accelerate the improvement of Zoysia japonica traits, tap the high-quality genetic resources of Zoysia japonica, and help researchers accelerate the cultivation of cold-resistant and herbicide-resistant Zoysia japonica varieties.
Agrobacterium-mediated transformation has apparent advantages in the genetic transformation of Zoysia japonica, such as relatively inexpensive cost and simpler operation procedures. In response to the existing problems in the genetic transformation of Zoysia japonica, we used the healing tissues obtained from Zoysia japonica grass species as the transgenic recipient materials, introduced the exogenous genes into the recipient cells with high efficiency through the improved Agrobacterium-mediated method, and obtained the transgenic regenerated plants with high induced regeneration frequency by selective cultivation, to establish a method for efficient genetic transformation of Zoysia japonica. At the same time, we introduced exogenous genes into Zoysia japonica healing tissues by selecting well-grown healing tissues, adjusting the composition of Agrobacterium floxed liquid medium and co-culture medium as well as the cultivation method, and improving the efficiency of genetic transformation through selection culture and regeneration-inducing culture. So far, we have helped researchers to effectively obtain herbicide-resistant Zoysia japonica strains and new cold-resistant varieties through the Agrobacterium transformation method.
Lifeasible provides professional, comprehensive, and reliable biotechnological support and services for scientific research institutions and plant researchers, and we have rich services in the fields of plant gene cloning, gene editing, vector construction, plant transgenesis, and so on. It is our original intention to promote the progress of plant science research together with our customers. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
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