Allium fistulosum Transformation

Allium fistulosum is an herbaceous plant of the Allium L. in the lily family, with a solitary, cylindrical underground bulb and a white, some with a slight light reddish-brown, outer skin of the bulb. The onion is cylindrical, hollow, and getting narrower from the bottom to the top. Allium fistulosum is native to Siberia and is now grown worldwide. Allium fistulosum has rich nutritional value and is especially widely loved.

Lifeasible is a scientific research and technology service enterprise based on gene function and gene industry application. We plant genetic transformation research and services; after years of development and technological research, we independently developed the Allium fistulosum genetic transformation and cell regeneration system, which can provide stable Allium fistulosum transgenic technology services for most scientific research workers.

What do we offer?

During the breeding process of Allium fistulosum, its seed propagation is limited by environmental factors, such as temperature, humidity and light, pests, diseases, etc. Therefore, genetic transformation research on Allium fistulosum is essential for mining genes regulating important Allium fistulosum traits, improving the appearance and flavor of Allium fistulosum, improving storage tolerance and stress tolerance, and breeding disease-resistant varieties. Appearance as well as flavor, improve storage and stress tolerance, and breed pest and disease resistant varieties. In the genetic transformation of Allium fistulosum, we often use young embryos, root tips, leaf blades, stem tips, and other tissues for direct transformation, mainly using the gene gun method and Agrobacterium-mediated method. The Agrobacterium-mediated method is currently the most widely used genetic transformation method, and the results are more satisfactory, and the technology is more mature. At the same time, we continue to improve the existing transformation methods, develop new transformation systems and methods, and establish an efficient transformation system with high transformation efficiency, good reproducibility, simplicity, rapidity, and wide adaptability so that the target genes can be integrated from random integration to directional localization. Multiple target genes can be introduced into the same receptor and entirely expressed in the transformed plants. At the same time, we have also removed or modified marker genes or bred transgenic plants without antibiotic marker genes and established a sound transgenic safety evaluation mechanism.

Notice to customers

• Customers can directly provide the constructed transformation vector or entrust us to carry out the relevant vector construction.
• Our company provides independent T0 generation seedlings by default, and the positive results of PCR identification of resistance genes are taken as the final standard.

Our experimental deliverables

• Each standard transformation provides 5-10 positive strains, or independent transformation strains can be provided according to customer requirements.
• Experimental data and pictures.
• Full lab report of the project.

Our service workflow

Why choose us?

• Systematic transformation system. Our years of research have led to developing a mature and commercially viable genetic transformation system for Allium fistulosum.
• High experimental success rate. We have been working on the regeneration and genetic transformation system of Allium fistulosum for a long time. Our technology is mature and stable, which can guarantee the success rate of experiments.
• Efficient experimental process. We can carry out genetic transformation programs at any time throughout the year. We utilize the Agrobacterium transformation method, which can carry out plant genetic transformation quickly, efficiently, and stably.
• Perfect service support. We provide complete technical support throughout the project.

Lifeasible is committed to plant genetic transformation research; we utilize the advanced transformation technology to provide various plant genetic transformation services, which are designed to meet the needs of researchers for plant genetic transformation research. We are committed to providing the most advanced technology and high-quality services to help researchers advance in plant research. If you are interested in us, please feel free to contact us.