Apium Graveolens L. Transformation

Apium graveolens L., commonly known as celery, is a perennial herb of the genus Celery in the Umbelliferae family. Celery is a vegetable widely grown throughout the world and is rich in dietary fiber, many vitamins, many mineral elements, and very beneficial ingredients for the human body. In addition, celery is rich in organic acids and apigenin, from which apigenin A and apigenin B can be isolated. Modern research has shown that apigenin has many medicinal uses, including lowering blood pressure, lowering blood lipids, preventing cancer, preventing atherosclerosis, and inhibiting bacteria. With the development of molecular biology, genetic engineering can compensate to a certain extent for the shortcomings of traditional breeding, but it also has the advantages of simple operation, high transformation rate, and short cycle time. The application of molecular biology techniques provides new tools for studying genetic functions and genetic improvement of celery.

What We Offer

Lifeasible is committed to providing you with a genetic transformation system for celery based on Agrobacterium transformation method with high stability, high transformation efficiency, high success rate, and short cycle time. Our one-stop custom transformation service is optimized by experts for years, including experimental design, vector construction, plasmid transformation, positive plant screening, and PCR identification. Agrobacterium-mediated transformation is known to be the best method for introducing genes into the celery genome. With rich experience in plant transformation, we have developed several different types of Agrobacterium-mediated gene transformation methods, such as GV3101, AGL-1, EHA101, EHA105, C58C1, etc. Moreover, for vector customization, we provide various vector structures, as shown below, for your choice.

Service Flow

1. Sample submission requirements

Customers can provide constructed celery transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

We have a standardized and scalable genetic transformation process for celery that allows us to provide targeted and customized services to our customers. All experimental procedures will follow strict quality control standards and be operated by experts who have been involved in plant genetics research for many years. Our staff will keep our customers informed by email when critical points of the experiments are available in case you miss them. In addition, we will also keep you informed of progress and data on a regular basis so that you are well informed of the experiment.

3. Apium graveolens L. transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• With superior expertise and high reliability, we are committed to ensuring the quality and success of each project.
• With our outstanding expertise in plant biotechnology, we are committed to full collaboration with leading research institutions and companies worldwide.
• We offer tailor-made solutions for celery transformation, providing a wide range of options for transformation methods, exosome types, Agrobacterium strains, and cloning vectors with various selection markers.
• We have a comprehensive pre-sales and after-sales service, including project consultation, session Q&A and seedling transplanting to ensure their survival.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Apium graveolens L. transformation. We can provide customized solutions to help you study a wide range of celery varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.