ATAC-Seq of Animal

Eukaryotic chromatin is composed of nucleosomes that form a bead-like structure and are further folded and packed. Transcription of genes requires that this higher structure be unpacked into a more loosely structured chromatin. This loose structure makes the regulatory elements (promoters, enhancers, etc.) on the DNA physically accessible. It thus can be bound by proteins such as transcription factors, activating the transcription process. ATAC-seq (assay for transposase-accessible chromatin with high throughput sequencing) is an epigenetic research technique for sequencing and analyzing the accessible nuclear chromatin region of Tn5 transposase.

Figure 1. Schematic diagram of the main steps of ATAC-Seq. (Grandi, F. C, et al. 2022)

Lifeasible uses Tn5 transposase to cleave the nuclear chromatin region developed at a specific time and space to obtain all actively transcribed regulatory sequences in the genome at that time and space and then clusters these sequences to mine potential binding transcription factors at these open loci by motif analysis, which is combined with gene expression level data to discover critical regulatory transcription factors.

Our ATAC-Seq Project for Animals

• Mouse ATAC-Seq
• Rat ATAC-Seq
• Pig ATAC-Seq
• Bovine ATAC-Seq
• Fish ATAC-Seq
• Marine Invertebrate ATAC-Seq
• Non-Human Primate ATAC-Seq
• Insect ATAC-Seq
• Equine ATAC-Seq

Our ATAC-Seq Service Process

Technical Parameter

• Sequencing platform and method: Illumina, BGI
• Sequencing strategy: PE150
• Sequencing data volume: 12 Gb Raw Data

Applications

• Mapping enhancers during development.
• Define genome-wide chromatin landscapes in diseases such as cancer.
• Mapping transcription factor binding sites and DNA methylation sites.
• Study the function of non-coding RNAs in the regulation of chromatin conformation.
• Identify cell-specific binding sites and transcription factors with cell-specific activity.
• Chromatin conformational changes and their mediated changes in gene expression before and after developmental processes or specific control treatments, etc.

Advantages of Our High-Throughput ATAC-Seq Service

• Low sample requirement: Only a relatively small number of cells are required.
• Wide range of applications: Suitable for chromatin accessibility analysis of most species and cell types.
• Frozen tissue compatible: Advanced technology makes frozen tissue ATAC-seq studies possible
• Rich analysis options: Rich bioinformatics analysis allows for various personalized analysis protocols.
• Simple experimental manipulation: No prior knowledge of epigenetic markers or transcription factors that control system dynamics is required.
• Convenient sample preparation: Freeze and store cells before submission, no need for fresh cells or isolated nuclei.

Lifeasible's ATAC-seq program has the advantage of "short cycle time, wide range, and high specificity" and has been widely used in the study of mice, rats, pigs, bovine, equines, fish, marine invertebrates, non-human primates, insects, and other species. It is our ideal to meet all the needs of our customers and to provide accurate and true data. We look forward to working with you; please feel free to contact us.