Benincasa hispida Transformation

Benincasa hispid, with its smooth flesh, unique flavor, rich in various vitamins and minerals, low-fat content, heat, diuretic and detoxification, and other health benefits, is a combination of delicious and health-care high-quality ingredients. Benincasa hispida, as a high-economic value crop, plays a vital role in the structural adjustment of the agricultural industry. Conventional breeding methods for Benincasa hispida are often limited by the lack of germplasm resources and the long breeding period, and genetic transformation research on Benincasa hispida can solve this problem to a certain extent.

Lifeasible has many years of experience in the genetic transformation of Benincasa hispida and can provide professional, convenient, and comprehensive technical support and services to research institutions and seed enterprises. We can provide comprehensive services in gene cloning, vector construction, plant genetic transformation, high-throughput screening, and assay analysis. In Benincasa hispida genetic transformation, we have established a sound genetic transformation technology platform, and we are committed to promoting the rapid development of Benincasa hispida research by screening the best transformation conditions and optimizing transformation solutions.

What do we offer?

The research on the genetic transformation of Cucurbitaceae plants has made significant progress, and we have introduced target genes with significant application value into Cucurbitaceae plants to achieve the purpose of improving varieties by continuously enhancing their transformation techniques and establishing efficient regeneration systems and genetic transformation systems. In this process, we apply advanced plant transformation technology, the process of obtaining transgenic plants by introducing exogenous genes into plant cells, tissues, or organs through genetic engineering principles and techniques. Currently, we mainly utilize the Agrobacterium transformation method for Benincasa hispida genetic transformation.

After years of research experiments, we have established and optimized the genetic transformation system of Benincasa hispida. In the genetic transformation of Benincasa hispida, we characterized Benincasa hispida as the material, cloned the target gene of Benincasa hispida by RT-PCR, and constructed the corresponding overexpression vector by Gateway homologous recombination, and then used the cotyledonary node of Benincasa hispida as the explant. cotyledonary nodes as explants and transformed using the Agrobacterium-mediated genetic transformation method. In this process, we screened and obtained the optimal medium for inducing adventitious shoots in cotyledonary nodes, the optimal medium for rooting, the optimal conditions for Agrobacterium infestation, the optimal antibiotics for bacterial inhibition, and other transformation conditions to ensure a high transformation rate of Benincasa hispida. Our technical support for the genetic transformation of Benincasa hispida plays an essential role in helping researchers screen for genes that can regulate important agronomic traits of Benincasa hispida and promote the cultivation of superior Benincasa hispida varieties.

Our research objectives

Benincasa hispida, genetic breeding technology, promotes Benincasa hispida new variety selection research; our research objectives according to the needs of different stages of production development have also changed, from the earliest from the simple pursuit of high yield, early maturity, disease resistance, and resistance to adversity, gradually upgraded to resistance to disease, tolerance to adversity, such as breeding resistance to blight, heat resistance, etc. Benincasa hispida genetic transformation research. The solutions and technical support we provide play an essential role in improving the quality and efficiency of the Benincasa hispida industry.

Note to Customers

• We are available all year round to conduct experiments on plant genetic transformation technology services.
• Customers can make personalized requests for project service options and delivery standards, and both parties can negotiate to determine the technical solutions and costs.
• Due to various factors, the cost of experiments may be adjusted from time to time, please consult us directly for details.

Project Reports We Provide

• Progress description.
• Sequencing report and pictures of vector construction.
• Pictures of explant invasion, induced healing, embryonic healing, tissue differentiation, plant regeneration, etc.
• Gel electrophoresis of PCR detection of target genes in T0 generation regenerated plants.

Our service workflow

Lifeasible has rich experience in the research and development of plant genetic transformation systems and has an advanced and comprehensive plant genetic transformation technology platform. So far, we have provided plant genetic transformation services for many research institutes, universities, enterprises, and individuals, and our transformation success rate is leading in the industry. If you are interested in us, please feel free to contact us.