Brassica Chinensis L. Transformation

Brassica chinensis L. is a herbaceous plant of the genus Brassica in the family cruciferae. The young leaves of brassica chinensis L. are edible as vegetables and are rich in vitamins, minerals, crude fiber, and carotenoids. Currently, the most prominent problem in the cultivation and production of brassica chinensis L. is the seriousness of insect pests. Pesticide usage is increasing year by year due to the increased resistance of pests, which not only increases production costs but also aggravates environmental pollution. The development of molecular biology provides new ideas for breeding insect-resistant varieties, which are difficult and have long breeding cycles. The introduction of exogenous insect resistance genes into plant cells through genetic engineering has provided a new way to improve brassica chinensis L. varieties. In addition to insect-resistant varieties, the development of disease-resistant, herbicide-resistant, salt-tolerant, cold-tolerant, and high-yielding varieties has also received increasing attention.

What We Offer

Lifeasible has a team of experts with many years of experience in plant genetic transformation and is committed to providing our customers with the most optimized, stable, and efficient transgenic services for brassica chinensis L. We have developed proven and customizable genetic transformation platforms for the targeted improvement and regulation of superior traits such as insect resistance, drought tolerance, and salt tolerance. Our genetic transformation system for brassica chinensis L. is based on the Agrobacterium transformation method, in which target genes are introduced into the recipient material through Agrobacterium infestation of Brassica campestris cotyledons. Eventually, the positive plants that meet the requirements are screened by DNA identification. The successful introduction of the target gene is also the only criterion for the success of the genetic transformation experiment, which is very important. We will eventually provide you with a detailed identification report and positive brassica chinensis L.seedlings that meet the requirements. In addition, our various gene modification services are shown in the following.

Service Flow

1. Sample submission requirements

Customers can provide constructed brassica chinensis L. transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Experienced experts are assigned to provide project consultation, experiment customization, vector construction, professional operation, and progress reporting throughout the entire process of genetic transformation services for brassica chinensis L.. The progress of the experiment will be updated regularly, especially the key points of the experiment, our staff will keep you informed by email. The success criteria for transgenics will be based on the PCR identification of the seedlings, and the customer must receive a positive seedling with a complete identification report. In addition, we will provide a complete after-sales service until the end.

3. Brassica chinensis L. transformation results delivery

• First, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We provide our customers with a scalable and versatile Brassica campestris genetic transformation platform that enables customization of the experimental process, covering experimental design, vector construction, plasmid transformation, positive identification, and resistance screening.
• Our experts have extensive experience conducting plant genetics research to ensure you are provided with reliable and professional services.
• Our genetic transformation services are based on Agrobacterium and can be applied to screening a wide range of resistances.
• Our Brassica campestris transformation experiments do not have any seasonal restrictions and can be carried out any time of the year.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in brassica chinensis L. transformation. We can provide customized solutions to help you study a wide range of brassica chinensis L. varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.