Brassica Oleracea var. Botrytis L. Transformation

Brassica oleracea var. botrytis L., also known as cauliflower, is a genus of Brassica in the cruciferous family. Cauliflower is a vegetable that is widely grown around the world and has high food value. Cauliflower contains vitamin A, vitamin B complex, niacin, vitamin C, protein, fat, carbohydrates, minerals, and beta-carotene, in addition to the anti-cancer substances quercetone, glutathione, indole, and isothiocyanate. Based on this, cauliflower has the effect of enhancing body metabolism, promoting detoxification, lowering blood sugar, lowering blood fat, and preventing cancer. To further improve the nutritional and industrial value of cauliflower, and reduce the cost of the cultivation process, genetic engineering breeding is increasingly utilized. Most of the genetic modifications are aimed at improving disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, and storage tolerance of cauliflower.

What We Offer

Lifeasible has established a cauliflower genetic transformation platform based on a well-established and standardized experimental process, which can provide customized and efficient transgenic cauliflower breeding services to our customers. Our versatile cauliflower genetic transformation platform is based on the Agrobacterium transformation method. In short, the entire process is carried out by experts by inducing embryonic tissue and co-culturing it with Agrobacterium, followed by stem and root regeneration to form complete cauliflower transgenic plants. To suit your various needs, we have a range of different types of Agrobacterium strains, including LBA4404, GV3101, EHA105, AGL1, C58, and GV2260. Our various genetic modification services are shown below.

Service Flow

1. Sample submission requirements

Customers can provide constructed cauliflower transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

All of our experimental operations and procedures are open to our customers so you can contact us at any time to follow up on the progress of your experiment. Our staff will also proactively report the results of your experiments to you at different stages of the experiment's progress. Our one-stop service aims to simplify your project research as much as possible, thus saving you time and costs for more important studies. All you need to do is submit your cauliflower samples as required and communicate your needs clearly to our experts, then leave the rest to us in trust.

3. Brassica oleracea var. botrytis L. transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We provide a customizable one-stop service covering all aspects of experimental design, vector construction, plasmid transformation, positive graft screening, and testing.
• To accommodate the different needs and uses of different customers, we provide different Agrobacterium strains and vectors with different selection markers for you to choose from.
• We have a professional technical team that has been engaged in cauliflower genetic transformation for many years, which can guarantee the success rate and high efficiency of the experiment.
• For common varieties, we have enough sterile seedlings ready for your project.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Brassica oleracea var. botrytis L. transformation. We can provide customized solutions to help you study a wide range of cauliflower varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.