Brassica Romanesco Broccoli Transformation

Brassica Romanesco Broccoli, commonly known as Romaine cauliflower, is a biennial herb of the Brassica species of the Brassicaceae family. Roman cauliflower is rich in vitamins, especially vitamin C. In addition, it also contains protein, fat, sugar, and minerals. Vitamin C can not only increase the body's immunity but also is a weight loss and beauty food that can fight cancer. The surface of the cauliflower bulb is composed of many spiral-shaped florets, which are arranged in pairs with the center of the bulb as the axis of symmetry. Its magic lies in its regular and unique shape, which has become a famous model of fractal geometry. Currently, efficient genetic engineering technology has become a hot topic in modern agricultural research. Driven by today's growing market demand, it is necessary to breed Romaine cauliflower varieties with high productivity and strong resistance to stress conditions.

What We Offer

Lifeasible has developed and established a mature Agrobacterium-mediated genetic transformation platform for Romaine cauliflower, where you can achieve improvement and customization of dominant traits. Based on this, we can provide our customers with short-cycle and high-efficiency genetic transformation services for C. rotunda, which will significantly facilitate your breeding research process. Our team of experts has been engaged in plant genetic transformation experiments for many years. It can fully meet the needs of our customers and further improve the efficiency of the whole transformation process with their proficiency. We have various of types of Agrobacterium strains (LBA4404, GV3101, EHA105, AGL1, C58, GV2260) and customized marker vectors (Kanamycin, Hygromycin, Phosphinothricin, G418) for you to choose from. This shows that the Romaine cauliflower genetic transformation platform is very flexible. In addition, the various gene modification services we offer can be viewed by clicking on the links below.

Service Flow

1. Sample submission requirements

Customers can provide constructed Romaine cauliflower transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our Romaine cauliflower genetic transformation service will equip you with an exclusive team of experienced experts, ensuring reliability, efficiency, and stability throughout the process. Before the start of the experiment, the experts are mainly responsible for technical advice and guidance work and assist customers in the optimization of the experimental process. After the experiment starts, we will report the project progress to you regularly so that the progress of the experiment is under your control. After the experiment is completed, we have a complete after-sales service and are responsible for guiding the transplanting of the seedlings to ensure their survival. All you need to do is to inform the experts in detail about your needs and confirm the experimental protocol, and then submit the samples as required.

3. Brassica Romanesco Broccoli transformation results in delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We use the Agrobacterium-mediated genetic transformation platform for Romaine cauliflower, which is fast, efficient, stable, and based on single-copy insertion and supports the screening of different bioresistances such as Chaumycin, G418, Basta, etc.
• We have complete service support during the pre, mid, and post experiments, such as project optimization, positive seedling transplanting guidance, after-sales service, etc.
• Our professional technical team has rich experience, which can greatly guarantee the high efficiency and success rate of the experiment.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Brassica Romanesco Broccoli transformation. We can provide customized solutions to help you study a wide range of Romaine cauliflower varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.