Camellia Oleifera Transformation

Camellia Oleifera Transformation

Camellia oleifera is a genus of Camellia and a woody oil tree species. Oil tea tree is known as one of the four major woody oil crops in the world, along with oil olive, oil palm, and coconut. The unsaturated fatty acid content of tea oil is much higher than that of vegetable oil, peanut oil, and soybean oil. The vitamin E content of tea oil is twice as high as that of olive oil. It contains specific physiologically active substances, such as camellia glycosides, which have high nutritional value. The development of the oil tea tree industry can greatly improve the efficiency of land resource use and guarantee the safety of food and oil. With the development of production and the increasing demand of society, people put forward higher and higher requirements on the yield, quality, and resistance of oil tea tree varieties, and the solution to these problems can no longer be satisfied by traditional breeding means alone. The emergence and development of transgenic technology have provided a new method for improving oil tea tree varieties.

Camellia Oleifera Transformation

What We Offer

After years of development and technical research, Lifeasible has a mature and high success rate of genetic transformation and cell regeneration system, which can provide stable transgenic technology service for customers of oil tea trees. Our experienced team of experts can work one-on-one to customize the program to meet the needs of our customers. Our oil tea tree transformation technology platform, using Agrobacterium mediated method, can complete the whole experimental process from vector construction to obtaining transgenic plants for you, helping you save valuable research time. Our wide range of Agrobacterium and selectable marker vector constructs ensure that your project needs are met. We offer the following range of constructing vector transformation methods for you to choose from.

Service Flow

Schematic diagram of the standardized process of oil tea tree genetic transformation. - Lifeasible

1. Sample submission requirements

Customers can provide constructed oil tea tree transformation vectors directly, and Lifeasible also offers vector construction services.

  • Customer-supplied plasmid requirements
Concentration Volume Other requirements
80-100 ng/μL ≥10 μL no degradation & no contamination
  • Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)
Bacterial broth Bacterial plate
Bacterial broth up to 1-year-old, preserved in glycerol Bacterial plate activated within one week
  • Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our experiments are open and transparent throughout the entire process, covering project consultation, experiment customization, vector construction, professional operation, and progress reporting. When the project reaches a critical point, we will inform you in time in case you miss it. Of course, you can also contact us at any time to get an immediate update on the progress of your experiment. Our one-stop service is designed to reduce your research burden and save time, so you can relax and focus on other more important aspects of your research.

3. Camellia oleifera transformation results delivery

  • First you will receive the T0 positive seedlings that meet your customized needs.
  • Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
  • Information about test primers, vector sequences, variant genes, etc.
  • Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
  • Complete, detailed and comprehensive experimental data and reports.

We Do Better

  • We have a team of technical professionals who have been involved in genetic transformation of oil tea tree for many years, which greatly ensures the success of the experiment and shorter cycle time.
  • Our oil tea tree genetic transformation services are backed by comprehensive technical support throughout the entire process, covering pre-project consultation, professional operation during the experiment and providing agreed results after the project is completed.
  • Our genetic transformation platform supports different types of resistance screening based on efficient, stable and mainstream Agrobacterium infestation methods.
  • Our oil tea tree genetic transformation services are not limited by season. For commonly used oil tea tree varieties, we have sufficient sterile seedlings to facilitate projects at any time.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in camellia oleifera transformation. We can provide customized solutions to help you study a wide range of oil tea tree varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.

For research or industrial raw materials, not for personal medical use!
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