Catalpa bungei Transformation

Catalpa bungei is a deciduous tree in the genus Catalpa of the family Zygophyllaceae. Catalpa bungei plays a vital role in industrial timber, garden ornamental, ecological protection, greening of the city. Catalpa bungei has an essential value of development and utilization. The natural breeding of Catalpa bungei has the problems of long nursery cycles and limited improvement of varieties, which causes a significant obstacle to the propagation and popularization of Catalpa bungei's excellent strains. By utilizing modern molecular biology and genetic engineering techniques, it is expected that the traits of the target species can be altered more quickly and efficiently, thus greatly accelerating the speed and efficiency of breeding.

Lifeasible is dedicated to the plant field, and we aim to utilize advanced biotechnology to provide researchers with efficient and reliable plant genetic transformation services. Using Catalpa bungei embryonic healing tissue as the recipient, we have developed and established an effective Catalpa bungei genetic transformation system after years of research, laying the foundation for future genetic improvement of Catalpa bungei traits.

What do we offer?

The Catalpa bungei transformation system that we have established uses embryonic healing tissue as the receptor, the transformation population of the infested material is large, the regeneration capacity of embryonic cells is strong, and the long-term and stable preservation of embryonic healing tissue in the successive generations can satisfy the needs of genetic transformation on an annual basis. Then we transformed Catalpa bungei embryonic healing tissue by infestation, and the transformation efficiency is high. We then transformed the Catalpa bungei embryonic healing tissue by infestation, which was highly efficient. In addition, we obtained the optimal conditions for genetic transformation through repeated experiments and then transferred the exogenous genes into the Catalpa bungei genome. The Catalpa bungei genetic transformation system we established can effectively obtain Catalpa bungei regeneration plants, shows high propagation efficiency, robust growth of transplanted plants, significantly shortens the breeding process, and improves breeding efficiency.

We mainly used embryonic healing tissues as explants for genetic transformation mediated by Agrobacterium obtained the optimal genetic transformation conditions through several experimental studies, and then transferred the exogenous genes into the Catalpa bungei genome. The genetic transformation system of Catalpa bungei provided by us can help researchers promote the development of molecular genetic improvement of Catalpa bungei together.

The contents of our services

• Acquisition and proliferation of genetic transformation receptors.
• Induction of cotyledon healing tissue.
• Induction and proliferation of embryonic healing tissue.
• Preparation of Agrobacterium tumefaciens carrying target genes.
• Genetic transformation of embryonic healing tissues.
• Plant regeneration and rooting.
• Detection of regenerated plants.
• Seedling and transplanting of transgenic plants.

Notice to customers

• Customers can directly provide the constructed Catalpa bungei transformation vector or entrust us to carry out the relevant vector construction service.
• If you need to do special varieties, the experiment time will be increased, and you will need to provide the corresponding varieties of seeds or seedlings.
• By default, we will provide independent T0 generation seedlings and the positive result of PCR identification of the resistance gene will be taken as the closing standard.

Our service workflow

Our technical advantages

• One-stop service. We provide a one-stop research service according to the characteristics of the customer's project, which helps customers save a lot of research time.
• Efficient experimental process. We have developed specific transformation methods for specific plant species to maximize the success rate of experiments.
• Mature genetic transformation system. After years of experimental research, we have established optimal transformation conditions to ensure the smooth progress of experiments.

Lifeasible is an industry leader in plant genetic transformation research, and we provide researchers with professional, reliable, and efficient technical support for plant genetic transformation. We have established a highly efficient genetic transformation system for Catalpa bungei using embryonic healing tissue as the recipient. If you are interested in us, please feel free to contact us.