Chenopodium Quinoa Willd. Transformation

Chenopodium Quinoa Willd. Transformation

Chenopodium quinoa willd. is an annual dicotyledonous plant of the genus Chenopodiaceae, whose seeds can be used as a grain. Quinoa is rich in proteins, vitamins, polyphenols, flavonoids, saponins, unsaturated fatty acids, and phytosterols, which is a high dietary fiber food with comprehensive nutrition. With the continuous human research on quinoa, quinoa has made some progress in genetic breeding. However, the progress of traditional breeding is slow, and the cycle time is long. These problems have seriously restricted the creation and promotion of new quinoa varieties. The development of genetic engineering has provided a solution for researching new quinoa varieties, which is more efficient and stable to achieve the improvement and targeted editing of genetic traits. Disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, storage tolerance, high yield, and high nutritional value are the key directions for trait improvement in quinoa.

Chenopodium Quinoa Willd. Transformation

What We Offer

Lifeasible offers professional plant genetic transformation solutions covering all steps, from experimental design to positive transgenic quinoa seedlings. The quinoa genetic transformation experiments are based on the Agrobacterium-mediated cotyledon dip method and are operated by experienced botanical experts throughout. Our experts introduce and integrate exogenous target genes into plant cells using Agrobacterium-mediated infiltration of fresh quinoa cotyledons, followed by regeneration of transformed quinoa plants with plant tissue culture. The successful introduction of the target gene is critical and crucial for the success of quinoa genetic transformation. We have professional PCR identification tools to screen positive plants for you to ensure the success of your project. We offer a variety of genetic engineering techniques for quinoa transformation as follows.

Service Flow

Schematic diagram of the standardized process of quinoa genetic transformation. - Lifeasible

1. Sample submission requirements

Customers can provide constructed quinoa transformation vectors directly, and Lifeasible also offers vector construction services.

  • Customer-supplied plasmid requirements
Concentration Volume Other requirements
80-100 ng/μL ≥10 μL no degradation & no contamination
  • Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)
Bacterial broth Bacterial plate
Bacterial broth up to 1-year-old, preserved in glycerol Bacterial plate activated within one week
  • Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our entire experimental process is standardized and scalable, allowing us to provide targeted transformation services for different traits. Our staff will keep our customers informed when quinoa genetic transformation experiments reach critical points and report to you regularly on the data and results at this stage. All experimental procedures will follow strict QC standards and be operated by experienced plant geneticists.

3. Chenopodium quinoa willd. Transformation results delivery

  • First, you will receive the T0 positive seedlings that meet your customized needs.
  • Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
  • Information about test primers, vector sequences, variant genes, etc.
  • Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
  • Complete, detailed, and comprehensive experimental data and reports.

We Do Better

  • We have a team of experts who have been working on plant genetics for many years and professional molecular experimental equipment to ensure a high success rate of genetic transformation and a shorter breeding cycle for quinoa.
  • The standardization and standardization of our experimental procedures and the possibility of individual customization allow us to do our best to meet your needs.
  • The advantage of a versatile transformation platform based on Agrobacterium-mediated transformation is that it is efficient, stable, and universal, while supporting the modification and improvement of different types of traits.
  • Our quinoa genetic transformation services can be offered at any time of the year, without the need for a specific season or time.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Chenopodium quinoa willd. transformation. We can provide customized solutions to help you study a wide range of quinoa varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.

For research or industrial raw materials, not for personal medical use!
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