Cloning Vector Building Service

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Cloning constructs are one of the most commonly used tools in molecular biology research. However, it is often time-consuming and laborious due to the high number of influencing factors and the requirement for researchers' experience.

Cloning vector building service – Lifeasible

With its rich experience in molecular biology, Lifeasible will concentrate our manpower to provide you with more efficient and faster services, including clone construction and its upstream and downstream related services as a complete solution. We will flexibly choose different cloning methods according to the source of target genes, including isolation of target genes from the genome, reverse transcription of specific mRNAs to synthesize cDNAs for cloning, and PCR in vitro amplification of target fragments for cloning.

Types	Details
Subcloning vectors	We have a wide range of high-throughput, low-cost cloning technologies that can target and seamlessly clone your target genes into the specified positions of the vector without restricting sequence and enzyme cutting sites. Construction of your desired full-length protein-coding ORFs with different tagged proteins and expression of functional fragments of proteins are available.
Plasmids	For molecular biology research, the quality of plasmids often determines the success or failure of experiments. We have professionals who have been working in molecular biology for a long time, and we can provide efficient, fast, and high-quality plasmid construction services for our customers. We can mutate single or multiple bases in the template plasmid for you according to your needs, and we can also subclone the specified fragments into each vector at the customers' request.
Sticky plasmids	We can construct a mucoadhesive plasmid from plasmid+cos sticky ends. The size is only a few kb, but the capacity of our constructs is 6-7 times its size, allowing the insertion of exogenous genes of this size to be replicated in the corresponding host bacteria.
Phage λ vectors	Phage λ must be modified to be used as a vector, and we can provide insertion-type and substitution-type vector-building services.

Advanced vector building services

Service flow

Cloning vector building service flow – Lifeasible

Cloning vector information

pUC19 vector. It is a small, high-copy number E. coli plasmid cloning vector. pUC19 encodes the N-terminal fragment of lacZa, which allows for α-complementation and pUC replication initiation. The plasmid is suitable for most commercially competent cells. The Amp resistance gene can be used to screen plasmids in E. coli.
pGEM-T vector. It is a highly efficient TA cloning vector of 3.0 kb size containing the Amp resistance gene for screening. The coding sequence is inserted by TA cloning, and the plasmid is suitable for most commercially competent cells.
pMD18-T simple vector. It is a highly efficient TA cloning vector constructed based on pUC18 with its initial MCS disrupted. The size is 2,692 bp and contains the Amp resistance gene for screening. The coding sequence was inserted by TA cloning at locus 425. The plasmid is suitable for most commercially competent cells.
pMD19-T vector. It is a highly efficient TA cloning vector constructed based on pUC19, with a size of 2.6 kb, containing the Amp resistance gene used for screening. The coding sequence was inserted by TA cloning at position 431.

Please feel free to contact our staff with your needs, and we will provide you with customized cloning vector building services and its related downstream solutions.

For research or industrial raw materials, not for personal medical use!

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