Cucurbita maxima Transformation

Cucurbita maxima is native to South America, and the fruit is sweet and nutritious. At present, the selection of new varieties of Cucurbita maxima is mainly based on conventional breeding methods, with a long breeding cycle and unstable genetic traits. With the development of genome sequencing and genetic engineering technology, genetic transformation technology to precisely improve plant traits can effectively accelerate the breeding cycle, providing new ideas for Cucurbita maxima germplasm resource innovation and variety selection.

Lifeasible is committed to plant genetic transformation research. Relying on the advanced plant transformation platform and experienced technical team, we aim to provide stable and rapid Cucurbita maxima genetic transformation services for many researchers and provide effective solutions to solve the problems of low transformation efficiency, poor reproducibility, false positives, etc. in the genetic transformation of Cucurbita maxima. and provide effective solutions to solve the problems of low transformation efficiency, poor reproducibility, and false positives in Cucurbita maxima genetic transformation.

What do we offer?

Cucurbita maxima genetic transformation technology is a significant bottleneck that constrains gene function mining and molecular breeding; after continuous attempts, we have developed a set of methods for efficient Cucurbita maxima transformation. In Cucurbita maxima genetic transformation, we mainly used the Agrobacterium-mediated method. We optimized the regeneration system of Cucurbita maxima in vitro by studying the regeneration system using Cucurbita maxima cotyledon nodes, hypocotyls, true leaves, and stem segments as the explants and establishing the effects of the culture method of aseptic seedlings, the age of seedlings, the size of the explants, and the ration of different phytohormone concentrations on the regeneration of Cucurbita maxima in vitro. We also studied the effects of varying bacteriostat concentrations, different antibiotic concentrations, ultrasound-assisted Agrobacterium-mediated method, and other factors on the genetic transformation of Cucurbita maxima in the regeneration of cotyledonary nodes of Cucurbita maxima. We established optimal regeneration and Agrobacterium-mediated genetic transformation conditions for Cucurbita maxima.

The Cucurbita maxima transformation services we provide can effectively help researchers improve Cucurbita maxima breeding. Currently, the Cucurbita maxima genetic transformation services we provide are effectively applied to the creation of breeding materials and the selection and breeding of new varieties, and we focus on comprehensive traits such as general adaptability, disease resistance, and storage and transportation resistance in addition to yield, quality, shape, and color.

Notice to customers

• Customers can directly provide the constructed transformation vector or entrust us to carry out vector construction.
• We provide independent T0 generation seedlings by default and take the positive result of resistance gene PCR identification as the closing standard.

Deliverables we provide

• Experimental data and pictures.
• Each standard transformation provides 5-10 positive strains, or independent transformation strains can be provided according to customer requirements.
• Experimental report of the project.

Our service workflow

Why choose us?

• High success rate. We have been developing plant regeneration and genetic transformation systems for a long time, and our technology is mature and stable, which can guarantee the success rate of the experiment.
• Efficient experimental process. We use an advanced plant transformation system, which can carry out genetic transformation projects anytime. We provide the Agrobacterium transformation method, which shows fast, efficient, and stable plant transformation.
• Perfect service support. We provide professional technical consultation and guidance to optimize the project and communicate at any time during the experiment to help customers keep abreast of the progress, as well as provide perfect after-sales service after the completion of the project.

Lifeasible is an industry leader in plant transformation; we provide efficient and stable plant genetic transformation services to maximize the satisfaction of customer research needs. If you are interested in us, please feel free to contact us.