Ciona Gene Editing

Aquatic organisms, especially large economic aquatic organisms, are an important source of high-quality protein for human beings, and it is not only of great scientific significance but also of great economic interest to carry out functional research on aquatic organisms' genes, reveal their genetic development laws, and explore and use genetic resources to breed high-yielding, disease-resistant and resilient varieties.

In the past decade, with the development of second-generation high-throughput sequencing technology, the genomes of nearly 100 species of aquatic organisms such as zebrafish, medaka, tilapia, amphioxus, Ciona, etc. have been sequenced, while the genomic data of many aquatic organisms will be sequenced every year in the future, which will provide researchers in aquatic biology with more abundant genetic information resources than ever before.

Figure 1. Rare Ciona intestinalis underwater scene.

With the progress of Ciona genomics, transcriptomics, and whole transcriptome research, the study of Ciona has also entered the post-genomic era, and the development of functional genome analysis has become a major scientific problem that scientists urgently need to solve.

CRISPR/Cas9 technology services

Ciona is a representative species of urochordate ascidians for elucidating genetic functions in the simplified chordate body. Lifeasible uses CRISPR technology in Ciona to achieve targeted knockout of genes, which realizes the idea of rapid screening of gene function in Ciona.

ZFN technology services

Figure 2. Lifeasible offers a ZFN technology service process.

ZFN are nucleases engineered to induce DNA double-strand breaks at the target sequence. It has been used as a tool for generating targeted mutations in the genomes of a wide range of organisms in animals and plants. Lifeasible can efficiently introduce heritable mutations into the Ciona genome using ZFN technology, which makes ZFN a powerful tool for knocking out Ciona genes.

TALEN technology services

TALEN electroporation results in high mutation rates, TALEN can efficiently cause tissue-specific knockouts, and Lifeasible's current protocol enables rapid analysis of hundreds of TALEN-induced mutations. For more information or any inquiry requirements, please contact Lifeasible.