Integration Sites Analysis

Flanking Sequence Analysis

Following the quick development and massive application of genetically modified organisms (GMOs), it is increasingly urgent to establish a reliable evaluation system to ensure the safety of GMO products. The expression of exogenous genes, which are incorporated into GMOs via different approaches, might generate varying outcomes due in large part to the differences in their genomic location, genetic structure and copy numbers. Therefore, the molecular characterization of exogenous genes is essential for the detection, labeling and safety assessment of GMO products.

The Flanking Sequence Analysis refers to the biotechnological approach for the characterization of transgene integration locus and copy numbers. Conventionally, flanking sequence analysis can be performed via traditional Polymerase Chain Reaction (PCR) methods. However, when it comes to high-throughput T-DNA insertion mutagenesis, the use of conventional PCR approaches is often limited by technical barriers. In this regard, the next-generation sequencing (NGS) technique serves as an ideal alternative for its full compatibility with high-throughput assays. So far, the advent of NGS has enabled detection of exogenous DNA insertion at the resolution of single nucleotide, allowing the locating of both incomplete and multiple integration events.

Lifeasible, as a leading plant biotechnology company with a rich history of plant genetics, molecular biology and bioinformatic studies, have long been providing plant genomic analysis services for our customers worldwide. For transgene analysis, we offer both traditional PCR approaches and the advanced NGS techniques for the detecting and locating of transgenes. Noticeably, we also offer featured one-stop services that covers the entire package of your project, from sample to data, to save you time, labor and money. Our services include the following steps:

• Plant sample preparation (plant cells, tissues, organs and whole plants are all accepted)
• Genomic DNA extraction
• Flanking Sequence Analysis
• Traditional PCR approaches including: inverse PCR, Thermal Asymmetric Interlaced PCR (TAIL-PCR), adaptor-ligated PCR or Restriction Site Extension PCR (RSE-PCR).
• NGS approach
• (Optional) Validating of transgene location using Fluorescent In Situ Hybridization (FISH)
• Data collecting, statistical analysis and reporting

Our experienced scientists and experts will work closely with you to make sure your project is given the best intellectual and technical support. Welcome to contact us for service details, questions or collaborations.